Limits in Hybridization 
21 
staminate Cinerea vine. From these bags one normal berry was taken in 
the fall. This berry contained three apparently normal seeds. These 
will be planted and the resultant seedlings will be watched with interest.* 
Rotundifolia-Munsoniana Hybrid X Vitis Cinerea 
In 1917 eight flower clusters on the variety Eden were bagged and 
later dusted with pollen from a staminate Cinerea vine. Ho fruit 
resulted from this work. 
Winchell-Rotundifolia Hybrid X Vitis Rotundifolia 
In 1917 two flower clusters on the Winchell-Rotundifolia hybrid of 
1912 were bagged and dusted with Rotundifolia pollen and one berry 
resulted. From this berry one seed was obtained. This seed was 
planted and a seedling resulted which will be more fully described in 
our next bulletin. In 1918 five flower clusters on the same vine were 
again bagged and dusted with pollen from Rotundifolia vines. Three 
berries set. Two of these berries were lost through the hands of irre¬ 
sponsible persons. From the third berry one seed was obtained. 
Several berries developed on this vine during this season as a result of 
the activities of natural agents of cross-pollination. Because this vine 
is completely self-sterile and is entirely surrounded by thousands of 
Rotundifolia seedlings and will cross with V. rotundifolia, and as the 
blooming period of this hybrid and that of the Rotundifolia vines prac¬ 
tically coincide, we assume that these berries, which developed under no 
human control, are the natural results of cross-pollination with Vitis 
rotundifolia. Three fruits of this type have been produced from which 
three seeds have been obtained. 
Vitis Rotundifolia X Herbemont-Rotundifolia Hybrid 
In 1916 two flower clusters on a seedling vine of Hope parentage were 
bagged and dusted with pollen from the Herbemont-Rotundifolia hybrid 
of 1913. Ho fruit set from this pollination. In 1917 eight flower clus¬ 
ters on Scuppernong vines, one on H-28, one on 0-23 and three on 
Eden were bagged and dusted with pollen from this Herbemont-Rotundi¬ 
folia vine. One fruit set on the Scuppernong vine, which yielded one 
seed. Ho fruits were obtained from any of the other cross-pollinations. 
Although very little fruit set, we have learned that Vitis rotundifolia 
will occasionally cross with its generation with V. bourquiniana. 
Vitis Rotundifolia X Vinifera-Rotundifolia Hybrid 
In 1918 four flower clusters on K-19 were bagged and dusted with 
pollen from the Vinifera-Rotundifolia hybrids of 1917 origin. Two 
* Since the writing of the manuscript for this bulletin one of these three seeds has 
germinated. This seedling from all appearances is a true Rotundifolia and therefore not 
of hybrid origin. 
