CONTENTS 
ABSTRACT. eoeeepeeeeeeeeeesee *@eeeoeeeeneweeoeeeeheeoeeeeaespeeeas eoeseawaeoeeeeeseoweeeaeeeseaesn 
INT RODUCTI ON eoeeseoteoaoeceeeeeseeeeeaeeeeseaanea oeeevoeoeveeweeneeeeeeoeseseeceeseeeeeeeeeoene 
PROCEDURES eeeeeesteeeoeseaoeseeoeeees eoeesseeeet#teeeeeaeeeeeenveeees *#spneseteoeoeeoeew#eeeseeweeeeesesvaeea 
Extraction and Concentration of Steroid Hormones....sessccceeccves 
Preparation of Chromatographic Columns.....scsseecccssssccccvces oe 
Separation of Steroid Hormones on Chromatographic Columns......... 
Visualization of Steroid Hormones on Chromatographic Columns...... 
Staining with iodine. .csseseesccseece Cree ewae nese teeeeeedes 
Charring with salfurtic -achds ¢s-0.so40e$ ese b-n a neaee od 8 ago eee ® 
Scanning of Absorption Peaks........ cb cede ceneen eS one Sew dieveptes 
Check of Recovery Using Radioactive Steroids. ..scescecccecesacsecs 
RIS Use e's 3 ak abies a} avd oe @eeee eoeeteetoeevoe@svwpaesevpeeeseeseeeveevesesevoeoeeeeneeeeeaseeoenenese 
Steroid Hormone Standards..... FC Pe ee eRe eh ea meee eereeeseeseueeeeene 
Chromatographic separation and sensitivity of detection...... 
Scanning of absorption PeaksSeereccceecccscceccscccceescsacecs 
Percentage recovery of radioactive hormones added to plasma.. 
Steroid Hormone UnknownsS....cecssescccacece Seeder se eces ees eeenees 
AVLAaN SpecLes. EXaMtiad . 2. cess e cd iwessespadeesepecdebereveeiias 
Identification of steroid hormone components from males and 
FOMALOS 60s oe sin Pee eee eee weed anes ota e be ene eae tiasaeateneees 
DISCUSS LON < 6: 5: dep -o.a prs wos 6.004 ease say grec elgg 0958's ore Ue wae ile ens oH ha ee AES oe 
Recommendations for Analyses of Steroid Hormone Unknowns...... <e4 9 
Authentication of Separatory ProcedureS..ssscesesccceccccccccceces 
Circulating Steroid Hormones in Females... .scccceccccccvccscsccecs 
Circulating Steroid Hormones in MaleS...cocscccscccccccssccecscces 
scanning oF AbsotptiLoi, Peake s.. cv ood o o0le else ses 4, slebeed need ed edess 
ACKNOWLEDGMENTS ....eeececs *eeeas eeseeeocoeoeeeeseeseeeeeoevoeeeeeeseeseeseeeeeseeseaeeeseneanee 
REFERENCES ..ccesecvae seesees seeneneeeeeae *eeeeteseetesees @eeeoeeeeweeteeeeeoeseeeeeeeea 
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