Scanning of Absorption Peaks 
The elution of individual bands and scanning of absorption peaks were 
not particularly useful in regard to distinguishing sex, but in the future 
this procedure promises to provide a means of positive identification of 
individual steroid hormones in different species. Circulating levels of ste- 
roid sex hormones should rise during the breeding season (Gilbert, 1971; Lake 
and Furr, 1971), and with higher concentrations, pooled extracts may yield 
levels high enough to quantitate by comparison with absorption peaks of stand- 
ards, or by fluorometric procedures. 
ACKNOWLEDGMENTS 
I wish to thank Dr. H. M. Ohlendorf, Dr. E. E. Klaas, Mr. S. N. Wiemeyer, 
and Dr. L. F. Stickel for permitting me to bleed their experimental birds. I 
am grateful to them and to Mr. J. R. Maestrelli and Mr. G. M. Sliker for 
helping me during the sampling procedures. I am also indebted to Dr. J. L. 
Ludke for conducting the radiochemical analyses. 
REFERENCES 
Bush, I. E. 
1961. The chromatography of steroids. Pages 191-196. Pergamon Press, 
London. 437 p. 
Gilbert, A. B. 
1971. The endocrine ovary in reproduction. Pages 1449-1468 in D. J. Bell 
and B. M. Freeman, eds. Physiology and biochemistry of the domestic fowl. 
Vol. 3. Academic Press, London and New York. 1488 p. 
Lake, P. E., and B. J. A. Furr. 
1971. The endocrine testis in reproduction. Pages 1469-1488 in D. J. Bell 
and B. M. Freeman, eds. Physiology and biochemistry of the domestic fowl. 
Vol. 3. Academic Press, London and New York. 1488 p. 
Randerath, K. 
1963. Thin-layer chromatography. Academic Press, London and New York. 
250 p. 
Ribi, E., C. J. Filz, G. Goode, 5. M. Strain, K. Yamamoto, S. C. Harris, and 
J. H. Simmons. 
1970. Chromatographic separation of steroid hormones by centrifugation 
through columns of microparticulate silica. Journal of Chromatographic 
Science 8: 577-580. 
Zaffaroni, A. 
1953. Micromethods for the analysis of adrenocortical steroids. Recent 
Progress in Hormone Research 8: 51-83. 
