Table 1.--Recovery® of !4c-testosterone and !'C-8-estradiol added to plasma 
samples from male or female Japanese quail. 
a nn nnn snide 
Samples Total counts on Total counts per Percent 
column (CPM) sample (CPM) recovery 
"testosterone" band 200 ,660 38.8 
517,400 
remainder of colum 1,090 0.2 
| "B-estradiol" band 101,580 13.5 
| 656,200 
remainder of colum 21,180 Ju? 
‘ _ = ee a 
Plasma samples were extracted with chloroform and chromatographed in ethyl 
acetate:cyclohexane (1:1 by volume). Separated bands were stained with iodine, 
cut off and oxidized, and counted in a liquid scintillation spectrometer. See 
"Procedures" for further details. 

—_—_—_S_—__* SX 
