20 
TESTOSTERONE 
| 30 
ESTRADIOL c 
CHOLESTEROL ,240 
5O| 

Figure 1.--Diagrammatic representation of migration rate (Rp) of authentic 
steroid hormones on silica gel columns. Columns contained 6 percent water 
and were packed for 5 min at 1500 G and developed for 7 min at 2500 G in an 
ethyl acetate:cyclohexane (1:1 by volume) solvent system. Steroid hormones 
were dissolved in chloroform:methanol (97:3 by volume) at a concentration of 
1 uwg/ul, and 10 pug of testosterone added to the first column, 10 ug of B- 
estradiol added to the second, and 10 ug of each, plus 10 yg of cholesterol 
added to the third colum. 

