ESTRADIOL 
ABSORBANCE 

200 300 400 500 600 
WAVELENGTH (nm) 
Figure 3.--Sulfuric acid chromogen spectra of varying amounts of authentic 
B-estradiol scanned from 190 - 600 nm at a speed of 100 nm/min and a chart 
speed of 2.54 cm/min. Full scale absorbance was set at 1.0. 
10 

