6 _sttestosterone 
Oa a estradiol 
ABSORBANCE 
CG) 

200 300 400 500 600 
WAVELENGTH (nm) 
Figure 5.--Sulfuric acid chromogen spectra of authentic testosterone and 
B-estradiol separated and eluted from the same silica gel colum. Solid line 
depicts scan of testosterone and dashed line that of B-estradiol. 
12 
