_ srl male 
female 
ABSORBANCE 

200 300 400 500 600 
WAVELENGTH (nm) 
Figure 6.--Sulfuric acid chromogen spectra of extracts from male and female 
Solid line depicts scan of eluate from male extract chroma- 
Japanese quail. 
from 
tographing with authentic testosterone, and dashed line that of eluate 
female extract chromatographing with authentic B-estradiol. 
13 
