On the day after the first spraying in the 0.8 area, small dead 
killifish were found in tide pools and some were noted in ditches. None 
could be seen in the large creek draining the area or along its banks. 
On the second day after spraying additional deaths in the tide pools were 
noted, and there was a beginning of mortality in the deeper pools. At the 
same time mortality in the ditches was evident but not heavy. Again no 
dead tish could be found along the creek bank. By the fifth day after 
spraying practically all evidences of fish mortality had disappeared, and 
no new deaths had taken place. The second spraying on this unit gave 
similar results. On the whole, the mortality in the 0.8 area was approx- 
imately half of that observed in the 1.6 area. 
In the 0.) area, not one dead fish could be found in the tide 
pools, ditches, or creeks on the second day after the first spraying. 
Searches made through the fourth day after spraying likewise showed no 
dead fish anywhere in the area. However, after the second spraying, light 
but widespread mortality and weakening of fish was evident. By the fourth 
day all such effects had disappeared. The difference in results of the 
first and second spraying may be due to the slightly greater dosage rate 
of the latter. 
A thorough survey on part of the 0.2 area one day after the first 
spraying showed only a few dead fish. These were in the shallowest pools 
and consisted of Fundulus no more than one inch long. On the two succeeding 
days, no new deaths were discovered, nor were any fish noted in a weakened 
condition. Results of the second spraying were similar. A few dead fish 
were evident in the tide pools on the first day following application but 
rone were seen in the creeks and ditches. On the second day, there were 
a few dead fish in the ditches and creeks as well as in the pools. By the 
fourth day all traces of mortality had disappeared. 
Enclosed Channel=-Section Studies 
The same sets of creek and ditch screen barriers used in evaluating 
DET damage to blue crabs (described under that section, page 15) also proved 
helpful in appraising the effects upon fish. However, this approach was 
applied only in the 0.) and 0.2 areas and was limited to the second spraying. 
The screen barrier in the upper part of each channel section was 
closed during early morning ebb tide of the day before spraying and the 
lower screen barrier was closed after the subsequent flood tide had progressed 
for about 3 hours. Following sprayings, dead fish in the enclosed channels 
were removed as found. When mortality had apparently ceased, the remaining 
live fish were recovered by poisoning with rotenone. 
The findings in the 290~foot channel section on the 0.l; area were 
as follows: | 
Dead Fish 2)32 
Live Fish 10 
Mortality 86% 
10 
