Ruelene (4-tert-butyl-2-chlorophenyl methyl methylphosphoroamidate) 
Excretion and blood data, obtained after sheep were given oral doses 
of ruelene-P32, indicated that ruelene is rapidly absorbed and excreted. 
Eighty-five to ninety-five per cent of the dose was recovered in the 
excreta. 
Analyses showed that this compound was degraded to inorganic phosphate 
which was subsequently incorporated in bone. After incubation of ruelene 
with whole blood and plasma, chromatography showed the presence of dimethyl- 
amido phosphoric acid and phenylmethyl phosphoric acid. Several other 
metabolites were found but not completely identified (Bauriedel and Swank, 
1962). 
Sheep and poultry hydrolyzed both ester and amide groupings and 
formed a large number of innocuous hydrolytic compounds that were excreted 
via urine of sheep or urine and feces of poultry. One compound from sheep 
urine was believed to be 4-t-butyl-2-chlorophenyl dihydrogen phosphoric acid. 
A compound was isolated from insects that had a similar Re (Brady and 
Arthur, 1962). 
Ruelene was fed to laying hens at a level of 100 p.p.m. for seven 
days. Six metabolites were isolated from excreta. Of these O-methyl - 
phosphoric acid and 4-tert-butyl-2-chlorophenyl methyl phosphoric acid 
were tentatively identified. Residues appeared in egg yolks 3 - 5 days 
after return to normal feed. Some tissues were residue-free after three 
days (Buttram, 1964). 
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