TID (Disulfiram) (Tetraethylthiuram disulfide) 
Plant tissues transformed N,N-dialkyldithiocarbamates into the 
corresponding glucosides (Kaslander et al., 1961) and alanine deriv- 
atives (Kaslander et al., 1962). By contrast, microorganisms produced 
aminobutyric acid derivatives (Sijpesteijn, 1962). 
In animals, enzymes converted TID to diethyldithiocarbamate, which 
in turn decomposed to CS5 (Johnston and Prickett, 1952; Prickett and 
Laug, 1953; Fischer, 1963). In man CS) was expired for 4-5 hours after 
treatment and about 50% of the administered TID was recovered as CS» 
(Merlevede and Casier, 1961). Ninety percent of an §39-labeled dose 
was absorbed from the intestinal tract and 10% was excreted in the feces. 
Analysis of urine indicated that about 65% of the sulfur was oxidized to 
sulfate and about 6% was recovered in the organic sulfur fraction 
(Eldjarn, 1950). Excretion of unchanged TTD in urine is practically 
nil (Domar et al., 1949; Linderholm and Berg, 1951); however small amounts 
of both changed and reduced TTD were recovered from urine (Eldjarn, 1950). 
The reduced form, diethyldithiocarbamic acid, has been detected in blood 
corpuscles and in alkaline urine but not in plasma (Linderholm and Berg, 
1951). Recently, N,N-diethylthiocarbamoyl -1-thio-6-glucopyranosiduronic 
acid was isolated from the combined urine of four men given oral doses of 
TTD. Identification followed paper chromatography, isolation, infrared 
analysis and comparison of the melting points of the isolated and synthetic 
triacetyl methyl esters (Kaslander, 1963). 
35 
Using S”~~-labeled material, a significant amount of the s35 was 
found attached to the serum proteins and to the soluble proteins of Liver 
170 
