21 
Sprayer and sprayed on a small field of wheat, in which the 
bugs were moderately plentiful. After over two weeks no ef- 
fect was visible. 
On June 18 about five acres of wheat were sprayed with the 
spores of the fungus in the following manner: a two-quart cul- 
ture was shaken and rubbed with water until it was mixed as 
well as possible. The spores did not mix very well but ap- 
peared to float as soon as the shaking was stopped. One or 
two jars of the mixture were then mixed with a barrel of water 
and this was pumped on the plots in asmall solid stream to make 
it reach the ground. A barrel of water was used to the acre. 
No visible effect followed this spraying, and the failure in the 
first attempt made it appear that the spores were not mixed 
thoroughly. A small amount of water was taken from the 
spray while in the field and from ita dilution-culture was made. 
Not a single growth appeared from this, showing the entire 
absence of spores in the water when it was pumped on the field, 
which was owing to their inability to mix with it. A turbid 
appearance of the water seemed to show the presence of quan- 
tities of spores, but this appearance undoubtedly came from 
the meal in the culture, the spores having been buoyed to the 
surface as with oil. Itis very likely that a mixture might be 
obtained by the use of milk or some viscid substance, as glu- 
cose or flour. 
During the time of both applications a severe drought pre- 
vailed;this was followed by some slightshowers, after which the 
disease appeared, killing off the bugs. 
On July 2 an attempt was made to spread the fungus by 
means of corn-meal. A sack of corn meal was mixed with wa- 
ter to the consistency of dough and cooked. The contents of 
six pint jars of the fungus were then mixed with it, and this 
mixture was carefully spread over about an acre of wheat in- 
fested with chinch-bugs. The weather, unfortunately, became 
very dry about this time, so that the fungus completely dried 
up. 
An experiment in propagating the disease in large infection 
boxes was made as follows: On July 30a large wooden in- 
fection box was placed in the cellar, and a layer of earth two 
inches in depth spread over the bottom. Several stalks of grow- 
ing corn were then laid on this, and a number of chinch-bugs 
