New YorK AG&ICULTURAL EXPERIMENT STATION. 1k 
pounds, neither was the storage or excretion of nitrogen appurently 
affected. No relation was found to exist between the nitrogen and 
phosphorus balances. Certain well defined and marked effects were 
observed, however. (See Tables 3 and Io.) 
In our attempts at transition from one ration to the other we soon 
learned that it was not possible to change the cow suddenly from a 
ration rich in phytin to one low in this compound and keep the 
animal in normal health. Such a change caused her to become 
seriously constipated and recourse to a purgative was necessary. 
When the change from the unwashed bran to the washed bran 
ration was made slowly the animal gave no evidence of serious dis- 
turbance of health and appetite but the feces always became much 
darker in color and much dryer. 
The removal of the phytin from the bran had the effect of 
materially diminishing the volume of urine. Marked changes in 
the amount and character of the milk secreted were also observed. 
The transition from the high-phytin, to the low-phytin, ration 
caused an increase in the volume of milk accompanied by very large 
decrease in its percentage of fat. As shown in Table 4, the maxi- 
mum decrease in fat amounted to I.4 per ct. and kept pace with 
the withdrawal of phytin from the ration. The total solids de- 
creased about .8 per ct. Table 4 also shows that the maximum 
decrease in the percentage of fat was not permanently maintained 
but that even during the feeding of the low phosphorus ration there 
was a slow restoration of the proportion of fat, although the per- 
centage did not rise to as high a point as was found during the 
feeding of the high phytin ration. (See Tables 4, 11 and 12.) 
6. Influence of the two rations upon production.—Although the 
increase in milk flow tended to counteract the decreased percentage 
of fat, nevertheless the total fat production in a given length of time 
was considerably lessened by the substitution of washed bran for the 
unwashed. Similar results did not obtain with the total solids and 
Casein, 366 ilable: 12.) 
7. Oestrum period.—lIt is a very suggestive fact that the oestrum 
appeared with this animal for the last time on June 12, a dis- 
turbance which followed a long continued feeding of the washed 
bran ration. Whether the cessation of this period was in any way 
connected with the removal of the phosphorus and its associated 
elements from the bran can be at present only a matter of theory. 
