74. Report oF DEPARTMENT OF ANIMAL HUSBANDRY OF THE 
EXPERIMENT II. 
This was a comparison of rations carrying varying quantities of 
nucleo-phosphorus, with minor differences in their phytin content. 
In the winter of 1904-1905, another experiment was carried on 
in continuation of the work described on the foregoing pages. As 
stated, our original plan involved mainly a study of the influence on 
milk secretion of rations rich and poor in nucleo-phosphorus. ‘This 
second experiment was planned, therefore, with especial reference 
to this phase of our general investigation. Its general procedure 
was very similar to that followed in the first experiment, viz., the 
feeding of two rations in different periods which differed in an 
emphatic way in their content of a particular class of phosphorus- 
bearing bodies. In this instance the variation was made with those 
phosphorus compounds insoluble in dilute hydrochloric acid, con- 
sisting mainly of the nucleo-proteids and nuclein. 
THE EXPERIMENTAL ANIMAL. 
This animal (Cow 2) was a vigorous grade Holstein weighing 
966 pounds, that was in good condition when placed in the experi- 
ment and was from one to two months advanced in the period of 
lactation. She manifested a vigorous appetite throughout the entire 
experiment and consumed daily all the food offered. Her health 
remained apparently unimpaired by the food and treatment which 
she received. | 
MANAGEMENT OF THE EXPERIMENT. 
The details as to the place where the experiment was conducted, 
the collection of milk and excreta, the sampling and analyses of the 
foods and excreta and the feeding of the rations, were in all re- 
spects quite like those of the first experiment. The fat in the milk 
was estimated by both the Babcock and the ether-extraction 
methods. The results by these two methods agreed so closely that 
the figures obtained by the Babcock method are the ones used. 
THE RATIONS. 
Variations in the supply of nucleo-phosphorus in the rations were 
accomplished in the following manner: A basal ration was com- 
pounded consisting of rice, washed bran and oat straw. To this 
was added germ oil meal in order to increase the supply of nucleo- 
phosphorus. This meal was found to be especially rich in forms of 
phosphorus insoluble in dilute hydrochloric acid. An estimation of 
the purin bases, after cleavage with dilute hydrochloric acid, showed 
