NEW YorRK AGRICULTURAL EXPERIMENT STATION. 95 
2. Through catabolic changes the phosphorus of the phytin and 
that of the unused digested nucleo bodies was reduced to inorganic 
combinations, and was excreted chiefly in the feces, though to a 
small extent in the urine. 
(The phosphorus of the urine is calculated wholly as inorganic. 
While we may not be justified in doing this, attempts to separate 
phytin or other organic forms of phosphorus from the urine by 
the methods employed in all the other work failed entirely. What- 
ever error is introduced by this method of calculation must cer- 
tainly be small.) Further evidence of catabolic metabolism of phos- 
phorus compounds is found in the fact that the inorganic phos- 
phates of the milk were from three to five times greater in quantity 
than the total amount of such compounds in the food. ‘The rise 
and fall in the amounts of outgoing phosphorus compounds oc- 
curred almost wholly with the inorganic salts found in the egesta. 
The organic phosphorus bodies of the egesta were but little affected, 
if at all, by the proportions of phosphorus compounds in the food. 
Variations in the phosphorus supply appeared not to modify the 
appropriation of this element by the milk. 
3. No relation whatever appears to exist between nitrogen excre- 
tion and phosphorus excretion. 
4. It is shown without question that the physiological effect of 
the two rations, due to the withdrawal from the bran of such com- 
pounds as were soluble in slightly acidulated water, differed to a 
marked degree. With the washed bran ration as compared with 
the one containing the unwashed bran, the following differences 
were observed. 
a. Drier and much firmer feces with the washed bran ration, 
accompanied by a constipated condition, requiring in some cases 
the use of a purgative. 
b. A marked disturbance of appetite (in Experiment 3) when 
a sudden change was made from the washed bran ration to the 
one containing the unwashed bran, indicating some specific physio- 
logical influence of the compound or compounds removed from the 
bran by leaching. 
c. A greatly reduced flow of urine following a change from the 
unwashed bran to the washed bran ration, the reverse taking place 
when a reverse change was made. 
