New YorRK AGRICULTURAL EXPERIMENT STATION. 97 
experiment was not entirely satisfactory. In no period was the 
phosphorus income equal to the outgo and it is not clear what 
“would have been the result had the two rations been as fully 
differentiated in their phosphorus content as was the case in experi- 
ments 1 and 3. However, there are indications, not to be ignored, 
that the two rations in the nucleo-proteid experiment had a some- 
what unlike effect upon the flow and composition of the milk. 
The other two points must be considered together. The problem 
here involved is whether, if phytin exerts the physiological effects 
observed, its influence is to be attributed to its acid phosphorus 
radical or to the basic portion, calcium, magnesium and potassium, 
or to the organic compound as an entity. The question was then 
raised in the progress of the work whether the withdrawal from 
the bran, by leaching, of the bases calcium, magnesium and potas- 
sium, might not so influence the supply of the compounds of these 
elements in the rations as to cause results that otherwise would be 
attributed to the phosphorus supply. 
Analyses of both the unwashed and washed bran show that the 
leaching removed the greater portion of the magnesium and potas- 
sium compounds and but very little of the calcium compounds. The 
following figures comfirm this statement: 
TABLE XXXVIII.—INoRGANIC CONSTITUENTS OF BRAN BEFORE AND 
AFTER LEACHING. 






P. CaQ: MgO. K,0. 
= a ee ue Ber Gh: Per ct. Per ct. Per ct. 
Wim ae a ee mettre nl. ed, 1.42 .182 894 1.58 
WAVE Ire oie) eh tea ae pal yh ee Oia A ae oP aE eee ede) ee ee al 1145 .380 .162 084 


The marked difference in the composition of the two kinds of 
bran, as indicated above, very naturally suggested the inquiry as 
to whether the supply of certain bases in the washed bran ration 
might not be insufficient. Consequently the amounts of the in- 
gested and outgoing calcium, magnesium and potassium oxides 
were determined for two periods in experiment 3, one March 14 
to 17, inclusive, during which time unwashed bran was fed; and 
March 30 to April 2, inclusive, this being part of a washed bran 
period. The results of this inquiry are given in the following 
tables: | 
7 
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