114 REPORT OF DEPARTMENT OF BACTERIOLOGY OF THE. 
tentional inoculation. The object of the first series was to show 
that the conditions in the flasks were such that the legume bacteria 
could make successful growth, while the second series was intended 
to measure the probability of the flasks becoming accidentally con- 
taminated during the process of manipulation. The flasks inocu- 
lated with legume bacteria uniformly produced both an abundant 
and a pure growth of the germ with which they had been seeded. 
This shows that the conditions were good for the development of 
any legume bacteria which might be present upon the cotton. The 
uninoculated flasks remained sterile, indicating that there was slight 
probability that any of the flasks became accidentally contaminated 
during the process of the test. 
Results of the examinations.—A test of each of the eighteen 
cultures was begun on November Io and a second test, using a 
second portion from each commercial package, was started Novem- 
ber 15, 1906. While each of these tests was carried through en- 
tirely separate the data are here combined into a single table, in order 
that the results may be more easily compared. 
TABLE J].—RESULTS OF THE EXAMINATION OF NITRO-CULTURES. 


No. of Series Became 
package.| legume. No. turbid. Remarks 
pel was AB ENT EA ENN Sed Sipe EE ee Se eae 
jE gr aie Alfalfa. ..¢. { 1 | Not in 7 days.| Sterile. 
2 | Not in 7 days.| Sterile. 
ora VS. oe ie Bacterial contamination, 30 per ct. Ps. 
: eee Alfalfa. ae koaee radicicola. 
} 2 ie GO CaAVSa.. tine Bacterial contamination, 20 per ct. Ps. 
| ; radicicola. 
Liars: italia weer f 1 | Not in 7 days.| Sterile. 
2 HS daysiun iat Yeast. 
EVs. eh Altaliar cen. 1 | Not in 7 days.| Sterile. 
D | “7 days trees «2 Yeast and molds. 
View es Adfalfas as. 1S) \daysceae nie Yeast and molds. 
PEN PH AatIiih ett eke | Molds and pink yeast. 
aN fd Bos Met tee Altaita. wis) oe 1 | Not in 7 days.} Sterile. 
Dla T ALY Ste hee iced Yeast and foreign bacteria. 
VII Crimson® <.... Lee Ada ysits eee Yeast. 
pees Clovetac. et Qe days santa. a eVeasctiaridemolds: 
VIII Crimson LH OVidayst ear bes Yeast. 
clover... 2 NASA YS <ish clots Yeast. 
1X Crimson EtG Cay Skee eek Pink yeast. 
iA neon cloveteaces Dr GbsdaySiwaw. ss lwlink yeasts 
x Crimson “ Leiio days Foreign bacteria. 
io eligi CLOVER site. 3 2 | 6days.......| Foreign bacteria and molds. 
XI Crimson UPN avert ey St ae Pink yeast and foreign bacteria. 
page's ClOvere-n a: 21 4 days Yeast and molds. 
XII Crimson 1aSvedays Molds. 
clover. a.2 Dal Osa YS hse Molds. 
1S eoydayoe ei Foreign bacteria and 5 per ct. Ps. radiei- 
XIII Vetch... 45% cola. 
° 2a As aS eee ets Foreign bacteria. 
XIV Vetch: 2.5: 14) 9 aves Bet. 40 per ct. Ps. radicicola, foreign bacteria. 
‘ : pes acted oY per et. Psa radicicola, foreign bacteria. 
AYS sa T6s.te oreign bacteria. 
XV..... Vetch...... rik eae eh eriee eee * rs Parr 
AVS ce case east and molds, 5 per ct. Ps. radaicicola. 
XVI. Vetch...... i Bier aaa ee ee sear Hea % 
AVS ene olds and foreign bacteria. 
XVIT Vetch...... " i bbe Pas Rea dire and yeast. 
AYS te ee olds. 
XVII Vetch...... 2M PS LGaysena ee Foreign bacteria, molds and yeast. 




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