New York AGRICULTURAL EXPERIMENT STATION. 
237 
Tarte VIII.— SHowrnc Amounts or HyprocHtoric Acip TAKEN UP AT 0O°C. 
BY CASEIN IN CONTACT WITH SOLUTIONS OF VARYING 
CONCENTRATION. 
eee —— ee 







Concentra-| Concentra- : 
PROPORTIONS tion of tion of Behe caae eee Ore Degree 
AND Duration acid in acid in rae a 1 a eacain f 
ArproxIMateE | of contact water casein : a - to cone’n | Solution 
NORMALITY (ee eet! (ce, N-1000 leon hs Peres of 
or Acip USED. acid in’ 1 ce.\acid in 1 gm. ° casein. 
solution). casein). by casein. BiAter, . 
N-2400 0 .4168 0 0 6) ee! Sa ee 
200 cc. 3 hrs . 2848 26.4 | lea 927. 0 
for 1 gram 6 hrs . 2683 29.7 35.6 110.6 0 
of casein 12 hrs 0.2400 35.4 42.4 147.7 0) 
24 hrs . 2406 Sone 42.3 146.4 0 
N-1000 0 .9986 0 Osos Se OTe Ser lb ee te 
200 cc. 15 min. 8221 35.3 Wied, 42.9 0 
for 45 min .8172 36.3 1822 44.4 (9) 
1 gram 14 hrs . 1565 48.4 24.2 64.4 0 
of 3 brs .6631 67.1 33.6 101.3 0 
casein 6 hrs -6313 73.4 36.7 116.2 0 
10 hrs .6112 Ciao 38.8 13133 1 
24 hrs .5915 81.4 40.7 142.8 1 
N-500 0 1.986 0 0 Ohl Etat 
200 cc. 15 min. 1.670 63.2 15.9 37.8 0 
for 45 min. 1.570 83.2 21.0 53.0 (0) 
1 gram 1% hrs 1.453 106.6 26.8 jose 0 
of 3 hrs 1.383 120.6 32.0 93.9 1 
casein 6 hrs. 1.182 160.8 40.6 136.2 1 
10 hrs. rel76 162.4 40.9 138.0 2 
24 hrs. 1.142 168.8 42.5 147.5 3 
N-125 0 7.905 0 0 Ode RR ete ee 2 
15 min. 5.331 257.4 32.6 48.2 0 
100 cc. 45 min 4.253 365.2 46.2 85.8 0 
ae 14 hrs 4.050 385.5 48.8 95.1 2 
2 5 hrs 3.744 416.1 52.6 1119 2 
1 gram — a a a | ee 
of 0 *7 993 0 (0) (0) eres 
” 12 hrs 3.306 468.7 58.6 141.8 3 
casein od hive rauice Virges { 60.1 { 150.8 4 
i 3.195 479.8 60.0 150.2 4 




The following statements summarize the results presented in 
Table VIII: 
(1) At o° C. casein takes up increasing amounts of acid for 
about 24 hours, when equilibrium appears to be practically estab- 
lished, as is shown by the fact that the curves in Fig. 7 approach 
a horizontal position when the abscissae denote that length of con- 
tact between acid and casein. 
(2) The final amount of acid taken up by 1 gram of casein 
varies in proportion to the concentration of the surrounding acid 
solution, as is shown by the 24-hour results in the fourth column. 
With the range of concentrations of hydrochloric acid employed, 
* Acid of 7.993 concentration was used for last two experiments of the N-125 series. 
The percentage of acid taken up from the N-125 solution is greater because only 100cc. 
of solution were used. The ratio reached (column 6) is about the same. 
