New York AGRICULTURAL EXPERIMENT STATION. 251 
The time at our disposal for the work on casein, however, did not 
allow us to carry out these desired details, but the results obtained 
appear to be sufficiently decisive and uniform to justify the con- 
clusions drawn. 
ACTION OF SULPHURIC ACID UPON CASEIN WITHOUT SOLUTION. 
The study of sulphuric acid was similar to that of hydrochloric, 
except that the temperature effect was not investigated in the case 
of sulphuric, while somewhat more attention was paid to the 
reversible character of the reaction. Because of the slighter solvent 
action of sulphuric acid, the experiments should be performed at 
room temperature. 
stant in all experiments. 
Agitation of the reacting mixtures was con- 
Taste XIV.— SHow1nc Amounts oF SULPHURIC AcID TAKEN UP BY CASEIN 
FROM N-125 SoLuTIoN aT 25°C. 
(Proportions: 100 cc. of acid for 1 gram of casein. ) 

Concentra- Concentra- Percentage Ratio of D 
tion of acid | tion of acid of acid ie cone’n acid st 
: in water in casein origina in casein . 
Duration of contact. (=cc. N—1000 | (=cc. N—1000 solution to en ee 
acid in 1 ce: | acid in gm. taken up cone’2 acid ae 
solution). casein). by casein. in water, 1} Cos¢!4. 
O 8.000 0 0 OC SF ae ce oa 
5 min. 3.490 451.0 56.4 129.2 0) 
45 min. ZIO22 597.8 7437 295.6 2, 
3 hrs. 1.971 602.9 one 305.9 3° 
6 hrs. 1.958 604.2 10.2) 308.4 4 







TABLE X V.— SHOWING AMOUNTS OF SULPHURIC ACID TAKEN UP BY CASEIN 
FROM N-500 SOLUTION AT 25°C. 
(Proportions: 200 cc. acid for I gram of casein.) 




Concentra- Concentra- Percentage Ratio of D 
tion of acid | tion of acid of acid in cone’n acid “ih 
2 in water in casein original in casein lati 
Duration of contact. | (=e, N-1000 | (=cc. N-1000| solution to cage 
acid in 1 cc. | acid in 1 gm. taken up conc’n acid ne 
solution). casein). by casein. in water. rae. 
0 2.015 0 0 Oude Aieees eto ce 
15 min 0.515 300.0 74.4 582 0 
45 min 0.495 304.0 75.4 614 1 
3 hrs 0.493 304.4 75.5 618 1 
6 hrs 0.489 BODme LT, 624 2 
12 hrs 0.491 304.8 75.6 622 Zz 




