New York AGRICULTURAL EXPERIMENT STATION. 259 
ACTION OF ACETIC ACID UPON CASEIN WITHOUT SOLUTION. 
The work with acetic acid includes experiments at 0° C. with 
N-500 and N-1000 solutions and at 25° C. with N-125 acid. The 
results are tabulated below. 
TABLE XX.— SHOWING AMoUNTS oF AceETIC ACID TAKEN UP BY CASEIN. 












Tw Ot ‘ HYHHo a) GH fetes) = tel ¢ ' Qa fs: fog | ' 
pO q oo 9 O.38 rp Pint ae “ 
te . 8 ceo? | eg8G | oss | eos | Se 
ie ch i caeegede ae paneer OCR UES 9) Okc gale Ge: 5 
- _ » F ~» 
SREB 5 Se Tea ee mer Sed Sd an aS 
6 Og e u os oar G im taed a1 S BHeQ Once a 
og a eh Ge pees WES Ge rne, aloe erg o3 a 
on a iS oe Ors ol Me Oe Os ba oles Sr He 
Cees § H . | 88095 | Bigs | PSGSP | Soon | SB 
a, a fas) i re thier ad Ps paatas aes t= Shier eg Sen ‘onl 
N-500 OC. 0 1.916 0 0 8) Oe ne 
100 ¢. c. OO” C. 6 hrs. 1.430 48.6 25.4 34.0 0 
for OFC: 12 hrs. 1.433 48.3 2552 Bone 0 
1 gram of O2'Ce 24 hrs. 1.420 49.6 25.9 34.9 0) 
casein. 
N-1000 (VEC. 0 990 0 OC) Aes | oe aan teas 
100 c.c Oss 6 hrs 749 24.1 24.3 S22 0 
for OVS L2ants: * 774 21.6 DAO 27.9 
1 gram of O2nGs 24 hrs. Biol 25.9 36.2 @) 
casein. 
N-125 250°C 0 8.000 0 0 Oe Vial: owes cee ee 
100 c. c. pda ek Ber 5 min. 6.805 11955 14.9 Vie6 0 
for ve LO. 45 min. 6.214 178.6 2203 28.7 1 
1 gram of PAG (CL ors: 5.923 207% aT 26.0 Saye Al 1 
casein. PAS KES 6 hrs. 5.677 PAP pee" 29.0 40.9 2 


— —-— 
Briefly summarized, the results embodied in the preceding table 
indicate that,— 
(1) The action of acetic acid with casein is in a general way 
similar to that of the other acids previously considered. 
(2) Acetic acid is taken up by casein in considerably smaller 
amounts than are the other acids, conditions being uniform. 
(3) A comparison of the action of casein with the acids studied 
is graphically shown in Fig. 15. The curves represent results 
obtained with N-500 solution in each case at 0° C. 
NON-ACTION OF CASEIN ON DILUTE SOLUTIONS OF NEUTRAL SALTS. 
In order to determine the effect of displacing the H* ion from 
solution, half-gram samples of casein were shaken with 50 cc. por- 
tions of the pure N-50 KCl solution used for standardizing the con- 
ductivity cells, the duration of shaking varying from 5 minutes to 
a 
*Evidently contamination of filtrate caused abnormally high result. Low conductivity 
of acetic acid renders dilute solutions very susceptible to change in conductivity by slight 
contamination, 
