Grams 
of 
casein. 
= 
on) 
SS He XH Re Se SR Re HS CO OC 
— 
I 
I 
O 
A201 
-335) 
.581 
O72 
.000 
.030 
.060 
.080 
. 100 
b120 
.140 
. 160 
.180 
. 200 
. 500 
. 800 
REPORT OF THE CHEMIST OF THE 
Linen: 
A 
222) 
5 
(106.0) 
75° 
59- 
5G. 
SBE 
55- 
54: 
54- 
54- 
54- 
54- 
54. 
54- 
54- 
54- 
O 
O 
eli 
boo ON 
FAWW BW AN UO H F 
% free 
NaOH from 
conductivity. 
100.00 
Sieizen) 
(29.05) 
12.00 
2200 
1. Ot 
0.50 
0.39 
0.28 
Oni7 
0.00 
Color of solu- 
tion with .. 
phenolphthalein. 
Red. 
Red. 
Red. 
Red. 
Red. 
Red. 
Red. 
Red. 
Red. 
Red. 
Red. 
Red. 
Red. 
Red. 
Slightly pink. 
Colorless. 
Colorless. 
The numbers in parentheses are graphically interpolated from 
the curve, and correspond to the point indicating the normal salt, 
according to Sjoqvist’s roughly approximate method. This normal 
salt, with no excess of casein present, appears about 29 per ct. 
hydrolyzed. 
column may be regarded as all due to hydrolysis. 
The free alkali in the data tabulated below this in the 
The per ct. of free NaOH in the third column is calculated by 
the formula usually employed for hydrolysis k, x + (1—x) k, =k, ; 
100 x = per ct. of free alkali: k, = conductivity of free alkali 2am 

k,—= conductivity of unhydrolyzed sodium caseinate = 54.4; k, == 
observed conductivity of experimental solution. 

The accuracy of 
the formula depends on that of the observation brought out pre- 
viously that the equivalent conductivities of the neutral and various 
acid sodium salts of casein are the same. 
The view that the sodium salts of casein hydrolyze was held by 
Laqueur and Sackur?! for the salts acid to phenolphthalein. Our 
* Beit. Chem. Physiol. Pathol., 3:194. 
1903. 
