56 REPORT OF THE BACTERIOLOGIST OF THE 
time, but' for all practical purposes I think the di-basic is the 
best to use. I am very truly yours, 
GEORGE T. MOORE, 
Physiologist and Algologist. 
While the formule of Dr. Moore have been most used, other 
combinations have been employed. For the sake of brevity and 
exactness these combinations are given below, accompanied by a 
laboratory number which has been found useful in our records. 
In this media record the digits signify the form of media, as 
bouillon, gelatin, or agar; the first decimal place gives the kind 
of sugar employed and the second decimal place is left for any 
other variation. Thus, 1.31 and 3.31 signify the Washington 
formula for bouillon and agar respectively. 
MeptIA USED IN INVESTIGATION. 
SF? 




Laboratory : 
number of COMPOSITION OF THE MEDIA, Reaction. 
media. 
1.00 5 gr. Beef extract—Liebig’s i 
5 gr. NaCl 1.5 per cent normal acid 
10 gr. Witte’s peptone to phenolthaline 
1000 gr. Distilled water 
1231 Ler eee ee monobasic 
ite Caren Soest muDnBtS Reaction not adjusted 
1000 gr. Distilled water | 
, | , 
1 bese 2 Same as above, using tap water | Reaction not adjusted 
1333 Same as above, using well water | Reaction not adjusted 
‘ ; ; | 
1.34 Same as above, using rain water from a cistern | Reaction not adjusted 
eu 5 gr. Beef extract—Liebig’s 
5 gr. NaC} ‘ 
10 gr. Peptone—Witte’s 1.5 per cent normal acid 
1000 gr. Distilled water to phenolthaline 
15 gr. Agar 
10 gr. Lactose 
3.31 1 gr. Potassium Phosphate—monobasie 
0.5 gr. Magnesium sulphate 
10 gr. Cane sugar No adjustment in reaction 
10 gr. Agar 
1000 gr. Distilled water 
3.32 Same as above, using tap water No adjustment in reaction 
3.34 Same as above, using rain water from a cistern | No adjustment in reaction 
