60 REportT OF ‘THE BACTERIOLOGIST OF THE 
DATES OF PREPARATION, RECEIPT AND EXAMINATION OF COMMERCIAL CULTURES. 


Label | W here Date on Date ‘ 
No. Legume. purchased. package. received. Examined. 
Drentesaies AIPA Rot eer Wack ne. ent 4-15-05 5- 4-05 | May 10, June 5, 6, 7,8 
Dube tect ATAU ae eis a Henderson .|. 4-15-05 5- 5-05 | May 10, June 5, 6, 7,8 
BR Alfalfan.c oss Thorburn... 4-15-05 5-— 5-05 | May 10, June 5, 6, 7,8 
Oh and cee Alfalfa: a, sons SSIZCL rh. 4-15-05 5- 6-05 | May 10, June 6, 7, 8.8 
Get occ bi Soy Bean..... Henderson . 1— 1-05 5— 5-05 | May 10, 19, 25. 
LO Sie os Soy Bean..... Salzer se she.30 1-— 1-05 5- 6-05 | May 10, 19, 25. 
Sie eae Soy Bean..... Thorburn... 1— 1-05 5— 5-05 i May.10;5119> 25: 
iV Rae Soy, beans isen!! VaekKi-. acme 1-"1-05 5-10-05 | May 10, 19, 25. 
16,286. Cow Pea...... Salzer..7..2: 4-15-05 5- 6-05 | May 10, June 13, 14. 
LU firetotetares » Cow Pea..... | Lhorburnic. . 2-15-05 5— 5-05 | May 10, June 13, 14. 
TS aes iews OOWwree Ga. tan Vick es oe. 1-15-05 5- 4-05 | May 10, June 13, 14. 
TOG SS « Cow Pea...... Henderson . 4-15-05 5- 5-05 | May 10; June 138, 14. 



METHOD OF MAKING THE TESTS. 
It will be seen that the packages for alfalfa were all of the same 
date and presumably were prepared from the same stock solution 
at the laboratory of the company. They accordingly served as a 
check upon the individual determinations. The same holds true 
of the cotton for soy beans as well as of two of the packages for 
cowpea. 
In making a test of the cultures about one-sixth of a pack- 
age of cotton was carefully transferred to a 300-450 cc. Erlen- 
meyer flask containing 100 cc. of sterile, peptone-free (1.31) 
bouillon. The proportion of cotton to liquid was larger than is 
called for in-the directions accompanying the package. On one 
occasion 200 cc. of fluid and larger amount of cotton was used. 
The packages for soy beans were tested at one date with bouillon 
from which the sugar was omitted. At one test of the packages 
for alfalfa the bouillon was carefully prepared from boiled water 
immediately before use but was not sterilized. 
In all of the tests after the first one of May 10, one-half per 
ct. ammonium phosphate was added in sterile solution at the 
end of twenty-four hours. 
Plates on the peptone-free (3.381) agar were usually prepared 
on the third day, but at times on the second or fourth day. In 
inoculating the plates a single transfer was made with a platinum 
loop from the flask to a tube of melted agar. In like manner 
transfers were made from this tube to a second and from the 
second to a third tube. The Petri dishes were enclosed in paper 
bags and kept at first at approximately 21° ©. (70° F.) and later 
at 25-28° ©. (77-82° F.). The plates were inspected frequently 
and usually recorded on the fifth and tenth days. 
