New York AGRICULTURAL EXPERIMENT STATION. 63 
ce. of freshly made, but not sterile, solution (1.381). On the 
second day the flask showed a good turbidity. Plates gave an 
abundant growth, but none of the colonies resembled Ps. radici- 
cola. 
‘From the results with the control flasks of June 5 it would 
seém fair to suppose that the conditions in the test flasks were 
'favorable for the development of Ps. radicicola. The results 
from the fiasks of June 6, 18, and 14 render it probable that the 
manipulation did not introduce enough contamination to inter- 
-fere with the growth of anything which may have been upon the 
cotton. Morever, the results with the flask of June 8 indicate 
that when the directions® are followed by the farmer, the produc- 
tion of turbidity on the second day is not proof that the develop- 
ment of Ps. radicicola is progressing satisfactorily. 
A CO-OPERATIVE TEST OF THE COMMERCIAL COTTON 
CULTURES. 
PLAN OF THE TEST. 
The uniform failure of the commercial packages to develop 
vigorous cultures of the desired organism when given a favorable 
opportunity was a surprising result. A proper regard for the 
right of the parties interested demanded that these results be 
carefully verified before they were published. Accordingly the 
situation was explained to a number of bacteriologists, all of 
whom were more or less familiar with the organism under dis- 
cussion, and they: kindly consented to make check determinations ° | 
of samples of cotton. 

®*DIRECTIONS FOR Usine Nirro-CuLtureE: To Prepare Solution.—Procure 
a jar or bottle that can be scalded out thoroughly. After cooling, fill with 
quantity of clean water named on package A and add the contents of pack- 
ages A and B, mixing until dissolved. Cover the jar and set away in a 
warm, shady place, avoiding a heat greater than 100° F. At the end of 
twenty-four hours add the contents of package C and allow to stand until 
the water turns cloudy. This will usually take place in from twenty-four 
to thirty-six hours, depending upon the temperature. If the water has been 
kept very cold, it will require longer to become milky. To Inoculate Seed. 
With the cloudy solution, prepared according to the above directions, 
thoroughly moisten the seed to be planted. Do not soak them. As soon 
as all the seed have been in contact with the solution, spread them out in 
a shady place to dry. When dry the seed can either be planted at once or 
kept on hand for several weeks without damage, provided they are kept dry 
and out of the sun. To any of the solution that may remain add one gallon 
of water and mix with sand or earth. This inoculated earth may then be 
spread over the field and be harrowed in, adding an extra quantity of Nitro- 
Culture to the soil. 
