64 REPORT OF THE BACTERIOLOGIST OF THE 
For this co-operative work six packages of cotton were pur- 
chased from J. M. Thorburn & Co., New York, representing 
three legumes, the packages for each legume bearing the same. 
date, and therefore presumably acting as a check upon the 
¥ 
determinations. 
DATES OF PREPARATION AND DaTE AND PLACE OF EXAMINATION OF CULTURES 
IN CO-OPERATIVE TEST. 
WHERE AND WHEN EXAMINED. 
Lab Date on 

No. LEGUME. package. 
mats Newark. mae pak A Detroit. 
DSi at Crimson Clover..| 5- 1-05 | July 12...| July 18..... Julyl8Fa52) vulyies 
20 aaa Crimson Clover..| 5-— 1-09 | July 12...) July 18..... Siulvel Serene July 28 
SU ade Japan Clover....| 4-15-05 | July 12...) July 18..... July 18.....| July 28 
33) eae ete Japan Clover....| 4-15-05 | July 12...) July 18..... July 18.....| July 28 
oes Wax Beans..... 2-15-05 | July 12...| July 18..... July Meee July 28 
Sadek s Wax Beans..... 2-15-05 | July 12...| July 18..... July 18.....} July 28 



Kach package of cotton to be tested was. divided into eight 
portions with sterile instruments, the work being done in a 
moistened Hansen inoculating chamber. Each portion of cotton 
was carefully wrapped in sheets of sterile parchment paper. Sets 
of chemicals were weighed from a common supply, which, with 
the exception of the sugar, were of the best grade obtainable 
from Eimer & Amend. Commercial granulated sugar’ was used. 
The packages of cotton and the chemicals were forwarded by 
express in sealed packages to avoid being tampered with in 
transit. ; 
The plan agreed upon was to dissolve the chemicals in dis- 
tilled water and sterilize, the di-basic ammonium phosphate being 
in a separate solution. The liquid in each test flask was to be | 
one-eighth that called for by the printed directions accompany- 
ing each commercial package of cotton. After adding the por- 
tion of cotton, the flask should be held as near thirty degrees 
centigrade as possible. At the end of twenty-four hours the 
sterile ammonium phosphate solution was to be added. 
According to the printed directions accompanying the cotton 
(See Footnote, page 63) the fluid should be used when it became 
milky at the end of two or three days. Plates were to be made. 
at this time to determine the character of the growth present. 
