New YorK AGRICULTURAL EXPERIMENT STATION. 65 
The fluid was also to be directly observed with the microscope. 
The medium for the plates was the same as that first placed in 
the flask with the addition of one per ct. agar. 
The portions of cotton and packages of chemicals were sent on 
July 15 to Dr. J. G. Lipman, Soil Chemist & Bacteriologist to 
the New Jersey Agricultural Experiment Station, New Bruns- 
wick, N. J.; Dr. E. M. Houghton, Bacteriologist & Pharmacolo- 
gist, Parke, Davis & Co., Detroit, Mich.; Prof. F. D. Chester, 
Bacteriologist of the Delaware Agricultural Experiment Station, 
Newark, Delaware; and Prof. C. E. Marshall, Bacteriologist & 
Hygienist of Michigan Agricultural College Experiment Station, 
Agricultural College, Michigan. 
GENEVA TEST. 
On July 12, the portions of cotton were placed in flasks of 
liquid and held at 28°-30° C. Owing to a mishap, there was no 
control flasks containing a stock culture of Ps. radicicola. After 
twenty-four hours the proper amount of sterile ammonium phos- 
phate solution was added. 
No turbidity was evident on July 14, but on July 15 there was 
a fair amount in No. 30 and No. 31, and plates were made from 
all the flasks. By July 17 turbidity had appeared in all the 
flasks except No. 28 and plates were again made from all the 
flasks. No turbidity appeared in No. 28 and by July 27 it was 
decidedly moldy. 
Crimson clover No. 28.—Microscopial examination on July 18 
failed to show any germ life. The plates have little growth 
except molds. The few colonies which appeared did not resemble 
Ps. radicicola. 
Crimson clover No. 29.—Turbidity was evident on July 17 and 
decided on July 18. Microscope showed yeast cells common and 
bacteria few and non-motile. Colonies on plates were of large 
yeast in most cases. There was only one suspicious colony and 
that was also a yeast. 
Japan clover No, 30.—Fair amount of turbidity July 15 and 
marked turbidity with bubbles of gas rising on July 19. Micro- 
scope showed yeast cells and large immotile rods fairly abundant 
and in about equal numbers. Both series of plates had abundant 
growth, a large portion of the colonies being pink yeast. About 
D 
