New York AGRICULTURAL EXPERIMENT STATION. 67 
culture 28 was still clear; 29 slightly cloudy; 30 turbid; 31 
cloudy; 32. clear; 33 strongly cloudy. Examination in hanging 
drop revealed nothing in 28; a few long irregular rods in 29; the 
B. megatherium-like organisms, and the long rods already noted, 
as well as some short motile rods in 30; a few yeast cells and 
short spore-bearing rods in 31; nothing in 32; small motile rods 
in 38. None of the organisms observed in any of the cultures 
resembled P. radicicola. 
“On July 24 the macroscopic appearance of the different cul- 
tures was practically unchanged, except that a small mold 
appeared in 28, Further examination failed to show the pres- 
ence of P. radicicola. 
“ Agar plates from 30 and 83 were prepared on July 22. On 
July 24 the colonies on these plates were well developed, and 
consisted of the organisms already noted in the hanging drop 
preparations from the corresponding cultures. Continued obser- 
vation for another week of both liquid cultures and plates, as 
well as the numerous hanging-drop preparations from different 
colonies failed to reveal the presence of P. radicicola. 
“It was safe to assume from the above evidence that there was 
no growth of P. radicicola under the conditions of the experi- 
ment, and that the failure of such growth to appear was due 
either to the absence of P. radicicola in the inoculating material, 
or to the unsuitability of the culture media used for the develop- 
ment of this organism. To check this matter a freshly isolated 
culture of P. radicicola from alfalfa tubercles, which made satis- 
factory growth on the agar used, was inoculated into one of the 
culture solutions. The latter was treated precisely as were the 
others. No sign of growth appeared at the end of three days 
and a further quantity of P. radicicola was therefore introduced 
into the solution. At the end of five days the latter was still 
clear and showed no sign of growth. 
“Tt thus appeared that the culture solutions used were not 
adapted to the growth of P. radicicola and that the failure of such 
growth to appear could not justly be attributed to the absence of 
P. radicicola in the inoculating material. The results are mani- 
festly inconclusive and it is believed that better results would 
have been obtained had sterile tapwater been used instead of 
distilled water. The latter was used, notwithstanding the mis- 
