New York AGRICULTURAL EXPERIMENT STATION. 69 © 
SYNOPSIS OF WORK ON SAMPLES OF INOCULATED MATERIAL FROM DR. HARDING, 
OF GENEVA, N. Y. 
By LL. T. CLarK. 
Prepared solutions as per directions, namely: 
SEU ALPS AES tesa ety ob whale Uieialete ale ote a UML, 18 gm. 
Peres Pprospnate,.(MOno ),. . 2,4). eee PA ~ 2 gm. 
PrrTeSrIMOHU I PUAtOr tact ved ce oc 5 pea le eco sss are Maat» 1 gm. 
PPABULLCUWALED! ose. ac. as ose aaah earw Sle Gra STE Vist ok 1900 Ce. 
This solution was put into 14-litre Florence flasks, 235 Ce. in 
five flasks and 118 Ce. in two flasks, to correspond to size of bits 
of cotton received, and the flasks properly sterilized. Opened 
packages containing samples of cotton very carefully, taking 
precautions to exclude dust and! other foreign material, and 
dropped the bits of cotton into the flasks of sterile solution, and 
labeled ilasks to correspond to numbers on packages. Set flasks 
in temperature of 28° C. Examined at the end of twenty-four 
hours, noted turbidity, and recorded same. At this recording, 
added enough sterile ammonium phosphate solution to give the 
solution in the flasks .5 per ct. of the pure salt, and then allowed 
to develop further. Examined flasks at intervals of twenty-four 
hours and recorded changes whenever such was necessary. 
At the end.of four days plated proper dilutions in agar (one 
loop from flask to first tube and two loops from first to second 
tube) from each flask. Defective plates (porcelain covers) 
rendered these results worthless. 
At the end of seven days another set of plates was made from 
which the final records were taken. To avoid any possible error 
in identifying the characteristic colony of P. radicicola, check 
flask and plates of P. radicicola from cow-pea (Migge,—tube cul- 
ture) was carried along parallel with the six from Geneva. 
Colonies appearing on the plates at the end of seven days were 
examined with low power, compared with check plate.from tube 
culture (Migge) and their purity recorded. 
For personal satisfaction the same dilutions as used above were 
plated from each flask in a special agar, upon which the true 
colony of P. radicicola can ‘be more easily identified. The results 
secured from these plates bore out the results already recorded 
