70 Rerort oF THE BACTERIOLOGIST OF THE 
so closely that such differences as existed could be readily attrib- 
uted to error of experiment. 
The detailed report of the cultures was as follows: 
“Oheck Flask inoculated with laboratory culture: Turbidity 
intense with some sediment at the end of three days. Plates 
showed characteristic colonies of P. radicicola and no contamina- 
tion. Culture pure. 
“ Orimson clover No. 28.—Turbidity slight at the end of twenty- 
four hours but not dense at the end of three days. Plates showed 
purity of culture approximately 50 per ct. 
“Crimson clover No. 29.—Turbidity slight at end of twenty- 
four hours and decided, but not dense, at the end of three days. 
Plates showed no characteristic colonies of P. radicicola. 
“ Japan clover No. 30.—Very faint turbidity at the end of 
twenty-four hours, which became very decided at the end of 
three days. Plates gave colonies uniform and true in character 
to P. radicicola. Culture pure. 
“ Japan clover No. 31.—¥Faint turbidity « at the end of twenty- 
four hours, which became decided at the end of three days. 
Plates showed colonies not uniform and few characteristic. Some 
yeast and many ‘off’ colonies. Culture impure. 
“ Wax bean No. 32.—No turbidity had appeared at the end of 
three days. Plates gave few colonies, 75 per ct. of which were 
yeasts and 25 per ct. P. radicicola. Culture weak and impure. 
“ Wax bean No. 33.—Decided turbidity at the end of three 
days. Plates gave numerous and uniform colonies of P. radict- 
cola and no contamination of ‘off’ germs. Culture pure.” 

SECOND DETROIT REPORT. 
A second set of cotton samples was forwarded to Dr. Hough- 
ton Oct. 4, and the following report received Nov. 10, 1905. 
SYNOPSIS OF WORK ON SAMPLES OF INOCULATING MATERIAL FROM DR. HARDING, 
GENEVA, N. Y.—SECOND SET OF DETERMINATIONS. OCTOBER 23, 1905. 
‘Received set of samples Oct. 6, 1905. Prepared culture solution 
as per Harding directions, namely: 
Cane Sugar ...... do bad 9. okt SR DSSRPNLESE CS hive ee ined eee 18 grams. 
Potassium Phosphate (Meno)\e sya. os est eee ee 2 grams. 
Mapnesiuin “Sipe le 'G.": aur eanmeemeeiyetae sss sz, oe 1 gram. 
Distilled “Waterson, one ee, 2 ai 1900 Ce. 
