(2 REPORT OF THE BACTERIOLOGIST OF THE 
Sample No, 80.—Milkiness faint in forty-eight hours. In 
creased after forty-eight hours when Ammonium Phosphate was 
added. First set of plates possessed a single colony of P. radict- 
cola, also a large number of yeast colonies. Organism isolated 
from the one characteristic colony proved to be vigorous and 
strong. | 
“Sample No. 31—Same as No. 30. Plates developed a great 
many yeasts and several colonies of bacteria. No P. radicicola 
present. 
“Sample No. 32. E SGI ian remained clear until tenth~- day 
when a faint milkiness appeared, due to a pink yeast. No P. 
radicicola. 
“Sample No. 33. 

Solution remained perfectly clear through- 
out. Plates and slides free from organisms. 
“Check I and IT.—Solution became milky at the end bf twenly- 
four hours, which condition continued to increase until it became 
opaque. Plates and slides showed pure culture of P. radicicola. 
“ Conclusions.—Two samples out of the six, No. 32 and No. 
3, were practically sterile and only single colony of the nitrify: 
ing organism, P. radicicola, could be detected, even when sub- 
mitting the solutions to dilution platings in two media. The 
yeast found so commonly in the samples proved to be a non-fer- 
menting organism of high vitality, producing a colony with a 
somewhat transparent margin and dirty, greenish-yelicw center 
ai first, later becoming dense and opaque with a raised, glisten- 
ing surface. From the fact that this organism was found iden- 
tical in samples Nos. 28, 29, 30 and 31, and in large numbers, 
leads us to suspect its origin in the original inoculating solution, 
or in the cotton which was impregnated. Its growth in nitro- 
gen free solutions produced a uniform milkinesss not unlike that 
af J’. radicicola, although slower and without the formation of 
pigment so common of the wild yeasts, hence its liability of 
being mistaken for a growth of P. radicicola. 
