[4 Report OF THE BACTERIOLOGIST OF THE 
Ber Test or Nrrro Currurss. II. 
Oct. 9 to 23, 1905. 
PLATES. SLIDES. 
P. radicicola. Strains—CENTRIFUGED SAMPLES, 
Flask. 
Hours 48. | Hours 78. | Hours 264. Yeasts. Bact. pena 
orms. 
28 0 0 0 + 0 0 
29 0 0 0 + + 0 
30 + 0 ee + + 3 
Single colony 
31 0 0 0 + + : + 
32 0 0 0 No micro-organisms 
33 0 0 0 No micro-organisms 
Check 
I + + + 0 + f- 
Check | 
II | + + + 0 + + 

“T am sending you herewith a complete report of our latest 
examination. I regret that our work here should not have 
checked up as closely as that of the other workers. Certain it 
is, great care was exercised in carrying out the work, and we are 
unable to satisfactorily explain the discrepancy. A few days 
ago, however, in making an examination of the distilled water 
we employed we found that it contains about one part of 
albuminoid ammonia to one million of water. This may have 
had a beneficial effect upon the culture media by influencing the 
growth of germs in the material that was sent in July. From 
our detailed report sent you in September, you will see that the 
cultures at best were not very vigorous, A little coaxing, as ~ 
would be exercised by a little ammonia in the culture media, 
may have been sufficient for us to obtain a growth, while, if it 
had been absent no growth would have occurred. This seems 
to me the most reasonable explanation of the discrepancy. It 
would be interesting to know just what conditions the material 
was kept under before the examinations were made.’® I do not 
recall just when you sent the first lot of material to me, but 
indistinctly remember that it was kept, after its receipt, for some 
time in a cool place, away from the sunlight, until my assistant, 
Mr. Clark, came the latter part of July. I can readily under- 
stand that, if the material was placed in the sunlight or even in 
The portions of cotton while at Geneva were inclosed in the paper boxes 
in which they were purchased and ‘stored in a dark corner of our dimly 
lighted culture room which is held at approximately 17° C. (65° F.). 
