New York AGRICULTURAL EXPERIMENT STATION. 75 
a warm room at that time of the year, the germs might die out 
even more quickly than if kept in a cool place where the tem- 
perature did not fluctuate too much. There seems to be no 
question but that! the cultures rapidly deteriorate. This is shown 
very conclusively in our two examinations.” 
NEWARK REPORT. 
The report of Prof. Chester is dated September 4, 1905. 
“ Fnclosed please find notes covering the examinations of the 
Nitro-cultures. The cultures are all innocent of Ps. radicicola 
except’ No. 33, Wax Bean, and even this was a very weak speci- 
men. I am sorry that I did not fully finish No. 31. I thought 
at first that this, like No. 30, was going to prove sterile, when, 
after six days, a slight growth appeared in the flasks. I was 
unable to conduct! the experiment from this point as I had made 
arrangements to leave for New York State. 
“I feel quite sure, however, that no living Ps. radicicola were 
present on the cotton, and that the growth was due to some for- 
eigner which develops very slowly in the nitrogen-poor medium. 
“The media were made in full accordance with your directions. 
“Medium 48 is the fluid medium for starting the culture, and 
48 agar, the latter plus 1 per ct. agar. I think, however, that 
1 per ct. agar is not quite enough, making it too soft; I prefer 
1.2 per ct. or 1.5 per ct.” 
The details of the work are as follows: 
“Crimson clover Nos. 28 and 29.—No growth in flasks in six 
days and no plates made. Conclusion: Cotton apparently sterile. 
“« Japan clover No. 30.—Turbidity strong at the end of two 
days. Microscopic examination showed long motile rods of the 
B. subtilis type, and short coiled chains of the B. ruminatus 
type, but none of the Ps. radicicola type. Plates were made on — 
48 agar and on plain agar. On medium 48 agar, colonies all of 
B. ruminatus (B. subtilis Gr.) No Ps. radicicola colonies. On 
plain agar colonies of B. subtilis and B. rwminatus. Conclusion: 
Cotton contains no living Ps. radicicola, and only spores of two 
members of the B. subtilis group, i.e.: B. subtilis and B. rumina- 
tus, both common terrestrial forms. 
“< Japan clover No. 31.—Owing to my departure from home, I 
was unable to make plates from this culture, which showed but 
