78 ReEporr OF 'THE BAC’TERIOLOGIST OF THE 
media. It should, however, be noted in this connection that in 
a Single case a quantity of this media did not appear to be a satis- 
factory substrate for growth, although Ps. radicicola grew upon 
it in some cases. The reason for this condition was not dis- 
covered. The experiment of Dr. Lipman was unfortunately a 
similar case, while Dr. Houghton and Prof. Chester, using the 
same chemicals but other samples of distilled water, obtained 
abundant growth with stock cultures of Ps. radicicola."*. 
REPETITION OF TESTS USING RAINWATER OR TAP- 
WATER. 
RETEST OF PACKAGES Nos. 2 To 19. 
In accord with Dr. Lipman’s suggestion (see page 68) it 
seemed best to continue the test, using water which would more 
nearly represent farm conditions. Media were prepared, using 
the same chemicals as before dissolved in our city water. The 
water is derived partly from Seneca lake and partly from springs. 
During sterilization a copious precipitate, accompanied by a 
marked and persistent milkiness was formed. On this account 
the solution was unfit for use. Practically the same result fol- 
lowed the use of the water from a local well. A similar precipita- 
tion was obtained at Dr. Marshall’s laboratory where an artesian 
water was used. The tap water at Dr. Lipman’s laboratory is 
derived from a number of small streams flowing partly over the 
red shaie formation. The precipitate formed in the use of the 
water does not interfere with the work since the sterile nutrient 
solutions become quite clear on standing. 
Rainwater was tried with better success. A slight milkiness 
appeared upon sterilization, but this almost completely disap- 
peared upon cooling. The amount of this turbidity varied slightly 
with samples from different sources. The fluid prepared in this 
way from rainwater was designated as 1.34 and the corresponding 
agar as 3.34. 
On August 9, portions of packages Nos. 2, 8, 4 and 5 were 
placed in sterile fluid 1.34. Turbidity developed less rapidly but 
was good in No. 3 and fair in Nos. 2, 4 and 5 at the end of three 
“See Prof. Chester’s report, page 75. 
