New YorK AGRICULTURAL EXPERIMENT STATION. 719 
days. The microscope showed yeasts to be most numerous in 
all but No. 2. Plates made on agar (38.34) gave abundant growth 
in all cases. One colony from No. 2 and three colonies from No. 
5 resembled Ps. radicicola. None similar was found on the 
plates from Nos. 3 and 4. 
On August 10, samples from packages 10 and 14 were likewise 
tested in sterile fluid (1.84). No turbidity had appeared at the 
end of five days and plates inoculated from the fluid produced 
practically no growth. 
Three very interesting check flasks were run at the same date. 
One flask contained the proper chemicals dissolved in clean well- 
water according to the directions on the commercial packages. 
A second flask of fluid was prepared in the same way except 
that clean rainwater was used. Neither flask was sterilized. 
At the end of two days both flasks had developed a good charac- 
teristic turbidity although neither flask had received either cotton 
or ammonium phosphate. 
The third check flask contained sterile fluid (1.84) and was 
inoculated with four drops of tapwater. This flask received its 
proper amount of sterile ammonium phosphate solution at the 
end of twenty-four hours. At this time a slight turbidity was 
evident and this became very decided by the end of the second 
day. Most interesting of all, plates from this flask showed a 
nearly pure culture of an organism producing colonies which 
very closely resembled Ps. radicicola. 
Another set of six flasks, each containing 150 Cc. of sterile 
(1.34) bouillon received 1, 2, 4, 8, 16, 20 drops of tapwater respec- 
tively. Each of the flasks became turbid within thirty-six hours. 
The agar plates from these flasks showed a large number of 
colonies resembling Ps. radicicola. 
On August 13, samples from Nos. 16, 17, 18 and 19 were placed 
in sterile fluid (1.34). No turbidity had appeared in Nos. 16 
and 19 at the end of three days, and plates failed to develop 
any colonies resembling Ps. radicicola. In flasks Nos. 17 and 18 
the turbidity was good at the end of three days when the plates 
were made. The growth on the plates was abundant and on 
those from No. 17 a few colonies resembled Ps. radicicola. None 
of the colonies from No. 18 resembled it. 
