S80 ~ Report oF THE BACTERIOLOGIST OF THE 
SECOND CO-OPERATIVE TEST. 
GENEVA TEST. 
August 8, portions of packages No. 28-33 were placed in the. 
appropriate amount of sterile fluid (1.34). Turbidity came on 
very promptly; Nos. 28, 30 and 31 developed a good turbidity 
by the end of two days, and Nos. 29, 32 and 33 at the end of 
three days. Microscopical examination showed the bacteria to 
be much more numerous than the yeasts in all cases except No. 
29. The plates showed the pink yeasts to be present in Nos. 30 
and 31. From Nos. 28, 30, 31 and 33 a few colonies resembled 
Ps. radicicola in gross appearance, but the germ was a plump 
granular bacillus. Pending a further determination of the char- 
acter of this germ, the cotton should be given the benefit of the 
doubt. No thoroughly typical colonies of Ps. radicicola were 
found. 
MICHIGAN AGRICULTURAL COLLEGE REPORT. 
This test was made at Dr. Marshall’s laboratory by his assist- 
ant Mr. W. G. Sackett. “ Dr. Marshall states that all the credit 
should be given to Mr. Sackett who has checked the work in every 
way possible and has done everything very carefully. 
Freshly caught rainwater was used in making the media and 
the chemicals were from .the same supply used in all of the 
codperative experiments. 
The portions of cotton were added to the sterile solution Octo- 
ber 20, sterile ammonium phosphate solution was added October 
21, and plates were made from all the flasks on October 22, 23 
and 25. 
All control flasks were inoculated with a freshly isolated cul- 
ture of Ps. radicicola (soy bean) and were kept under precisely 
the same conditions as the cultures in question. These showed 
a distinct turbidity after forty-eight hours and plates made from 
the same developed true Ps. radicicola colonies. | 
The detailed report on the various samples follows: 
“Crimson clover No, 28.—No turbidity developed by October 
25. No growth on any of the plates. 
“Crimson clover No. 29.—Slight turbidity October 25. Micro- 
Scope showed many yeasts. Many colonies of yeasts appeared 
upon the plates but none of bacteria. . 
