New YorRK AGRICULTURAL EXPERIMENT STATION. Sl 
“ Japan clover No. 30.—Solution became turbid October 23. 
Microscope showed many yeasts but no bacteria. Plates showed 
many yeast colonies, a part of which were pink. No bacterial 
colonies appeared. 
“ Japan clover No. 31.—Turbidity was slight on October 23, and 
decided on October 25th. Microscope showed many yeasts and 
no bacteria. Plates developed many colonies of yeasts but none 
of bacteria. 
“Wax bean No. 32 and 33.—Flocculent turbidity appeared in 
each flask October 25. Microscope showed these to be masses of 
a short plump bacillus. No yeasts appeared upon the plates and 
but few bacterial colonies.” 
SECOND NEW BRUNSWICK REPORT. — 
A second set of samples of cotton and chemicals was furnished 
to Dr. Lipman who presented the following report October 30, 
1905: 
“The cotton cultures, 28, 29, 30, 31, 32 and 33 received on 
October 23, were inoculated into the nutrient solution made up 
according to the directions and all the solutions were made up 
with tapwater. As a check on these cultures, I also inoculated 
into a sterile portion of the nutrient solution, a pure culture of 
P. radicicola, isolated from soy bean tubercles, and sent to me 
by Mr. Karl Kellermann. All of the cultures were kept in the 
incubator at 27° for three days, and for three additional days 
after that at room temperature. The check culture showed cloudi-— 
ness at the end’ of eighteen hours even before the addition of the 
ammonium phosphate, and was strongly turbid at the end of 
forty-eight hours. The six cultures were all clear at that time, 
and showed no growth. At the end of three days, cultures 30 
and 31 were slightly cloudy; the others still clear. At the end 
of six days, 28, 29, 82 and 33 were still clear, while 30 and 31 
were cloudy. The latter, when examined under the microscope, 
contained medium-sized spore-bearing bacilli, and clostridia. It 
was not found necessary, in view of the above, to prepare plates 
from any of the cultures. These observations taken together 
with those previously reported to you, lead me to the belief that 
the cotton cultures: 28, 29, 30, 31, 32 and 33 contained no P. 
radicicola organisms capable of development under the conditions 
prescribed by the manufacturers.” 
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