254 REPORT OF THE CHEMIST OF THE 
TABLE I.—AMoUNT OF HypRocLoric AciIp NEUTRALIZD BY ONE GRAM OF 
BAse-F REE CASEIN Wirn CoNSTANT AMOUNT OF ACID. 
PREPARATION 3. 







No. of aya PREPARATION 1. PREPARATION 2. PREPARATION 4, 
‘ t’gt 
ce. of wy |e a | 
acid iS oe n-100 | n-100 | n-100 | n-100 | n-100 | n-100 | n-100 | n-100 
used. <4 HClin | HClin | HClin | HClin | HClin } HClin | HClin | HClin 
filtrate. | proteid. | filtrate. | proteid. | filtrate. | proteid. | filtrate. | proteid. 
cc. cc. ce. ce. ce. cc. ce. ce. 
40 n-200 13.6 6.4 13.6 6.4 13.8 6.2 14.0 6.0 
20 n-100 13.4 6.6 14.2 5.8 14.0 6.0 14.2 5.8 
16 n- 80 UES, 6.4 13.6 6.4 13-6 6.4 14.4 5.6 
12 n- 60 14.0 6.0 13.6 6.4 14.0 6.0 14.0 6.0 
8 n+ 40 14.0 6.0. 14.0 6.0 14.0 6.0 14.0 6.0 
4 n- 20 13.6 6.4 14.0 6.0 14.0 6.0 14.2 5.8 
Av’ge 137 6.3 13.8 6:2 13.9 Deal 14.1 5.9 



Amount of acid used varying in relation to proteid.—In an- 
other set of experiments, we used varying quantities of acid for 
one gram of proteid. Otherwise the experiment was carried out 
like those preceding. The results are given in Table II. 
TABLE JJ.—AMoUNT oF HypROocLOoRIC AcID NEUTRALIZD By ONE GRAM OF 
BASE-I'REE CASEIN, TE AMOUNTS OF ACID VARYING. 







Ss BN Be PREPARATION 1.| PREPARATION 2.¥| PREPARATION 3. | PREPARATION 4, 
$ 3 |adge 
$3| a2 |esS cra Set 7 
wie ee San 
Sy a4 |4 *>s| n-100 n-100 n-100 n-100 |} n-100 n-100 n-100 | n-100 
is ae a HClin | HClin} HClin} HClin| HClin | HClin | HClin | HClin 
s S| ns |g 44 \filtrate. |proteid | filtrate.) proteid.) filtrate. | proteid. | filtrate. | proteid. 
a 
eC; ce. cc. Ce cc ice, cc CEs ce. 
30 | n-200| 15 8.8 6.2 9.2 5.8 8.6 6.4 8.8 6.2 
20 | n-100] 20 oe 6.4 13.2 6.8 13.4 6.6 13.6 6.4 
20 | n- 80| 25 19.2 5.8 19.2 5.8 19.0 6.0 19.6 5.4 
10 | n- 60| 16.6; 10.8 5.8 10.6 6.0 10 8 5.8 1120 5.6 
AWVOrah 7.064. F sts oo oes 6 Ogi ateeret test Giese eats 62 2\- o ecte 5.9 



Amount of acid and amount of dilution varied in relation 
to proteid.—The amount of water in which the solid base-free 
casein was suspended was relatively considerable in the preced- 
ing experiments. We wished to see what difference if any, there 
might be when we treated the casein directly with acids of varying 
strength and without farther dilution. We suspended one gram: 
of the base-free casein in each of the following amounts of solu- 
tions of hydrochloric 
cubie 
centimeters of —2 
——— 
100? 
cubic centimeters of =. 
acid: Wifty cubic centimeters of —, 40 
30. cubie centimeters of 
a, Anda 
& 0 
The mixtures were allowed to stand 
one hour with occasional agitation and were then filtered and 
