New YorK AGRICULTURAL EXPERIMENT STATION. 261 
Preparation of basic calcium paracasein.—We dissolved about 
two grams of our paracasein preparation in lime-water and made | 
the solution neutral to phenolphthalein by addition of —~ hydro- 
chloric acid. The solution became more milky in appearance but 
no precipitation was observable. This solution was now pre: 
cipitated by alcohol, filtered, washed several times with alcohol, 
finally with ether and dried at 100° C. The preparation was 
insoluble in dilute salt solution and practically so in hot dilute 
alcohol. Determinations of calcium and chlorine gave the fol- 
lowing results: One gram contained a total of .0283 gram of 
CaO, and .0063 gram of Cl (equivalent to .0047 gram of CaO 
combined as calcium chloride). Deducting the calcium oxide 
equivalent, present as chloride, from the total, we have .0236. 
gram, which represents the amount of calcium oxide combined 
with the proteid, or 2.86 per-ct. This preparation therefore corre- 
sponds very closely in its calcium content, with the basic cal- 
cium casein. AS in the case of the calcium casein prepara- 
tions, all the calcium paracasein preparations contained calcium 
chloride. | 
Preparation of neutral calcium paracascin.—When we treat a 
lime-water solution of paracasein with an amount of acid suffi- 
cient to turn blue litmus red, or just a little short of this, the 
proteid separates as a precipitate and this occurs before all the 
calcium of the original lime-water solution has been neutralized. 
This product is insoluble in dilute salt solution and nearly insol- 
uble in hot dilute alcchol. The product was thrown on a filter 
-and washed several times with water. Filtration was rather _ 
slow. The precipitate was then washed with alcohol and ether 
and dried at 100° C. Determinations of calcium and chlorine 
gave the following results: One gram contained .0391 gram of 
ash, a total lime content of .0194 gram of CaO and .0(064 gram 
of Cl (equivalent to .0048 gram of CaO combined as calcium 
chloride). Deducting the CaO equivalent, present as chloride, 
from the total CaO, we have .0146 gram, which represents the 
- amount of calcium oxide combined with the proteid or 1.46 per 
et. This preparation therefore corresponds closely to the neu- 
tral calcium casein. | 
We have seen above that the neutral calcium paracasein. 
formed by making a lime-water solution of paracasein neutral 
