New York AGRICULTURAL EXPERIMENT STATION. 263 
minations of calcium and chlorine showed their presence in the 
proportions found in calcium chloride, which was probably held 
mechanically in the proteid mass. 
Behavior of lane-water solutions of paracasein convpounds to- 
wards rennet and soluble caletwm salts.—Basic calcium paraca- 
sein, prepared by dissolving hase-free paracasein in lime-water 
and making neutral to phenolphthalein, is not coagulated by 
rennet in the presence or absence of soluble calcium salts. - How- 
ever, a Solution of basic calcium paracasein may be coagulated on 
warming by soluble calcium salts alone without rennet. 
Neutral calcium paracasein, prepared by dissolving base-free 
paracasein in lime-water and making neutral to litmus, is coagu- 
lated readily at room temperature or on warming by soluble cal- 
cium salts, with or without rennet, but not in the absence of solu- 
ble calcium salts. | 
Rennet changes calcium casein to calcium paracasein but does 
not coagulate the proteid. Soluble calcium salts coagulated neu- 
tral calcium paracasein and the action takes place through quite 
a range of temperature ; while basic calcium paracasein is coagu- 
lated by soluble calcium salts only after warming. Here, as in 
the case of the corresponding calcium casein compounds, the 
coagulation may be the result of purely physical change or there 
may be a loose combination between the soluble calcium salt and 
the calcium paracasein compound. . 
Sunuinary of results of work done on paracasein and its com- 
pounds.—We have made and studied the following paracasein 
preparations: (1) Base-free paracasein, the free proteid; (2) 
basic calcium paracasein, containing about 2.40 per ct. of calcium 
oxide, and (3) neutral calcium paracasein, containing about 1.50 
per ct. of calcium oxide. | 
THE RELATION OF THE SALT-SOLUBLE COMPOUND OF PARACASEIN 
TO PARACASEIN. 
Of the three paracasein preparations made and studied by us, 
we have found only one that was readily soluble in warm 5 per 
ct. salt solution and hot 50 per ct. alcohol, and this was the base- 
free paracasein. This body on warming also showed the peculiar 
plastic property and the power of being drawn into long, fine, 
silky threads, which are shown by the salt-soluble substance pre- 
