268 REPORT OF THE CHEMIST OF THE 
chloride was removed. The precipitate was then washed with 
alcohol and ether, and dried at 100° C. The product was easily 
powdered. It contained 1.37 per ct. of ash. . 
BEHAVIOR OF SALT-SOLUBLE AND ALCOHOL-SOLUBLE PREPARATIONS 
TOWARD LIME WATER. 
We made a study of the power of these two preparations to 
combine with calcium in comparison with base-free casein, in 
order to ascertain whether they were base-free or not. Our 
method was the following: In portions of 50 ce. each of lime 
water of known strength, we dissolved .5 gram each of base-free 
casein, of the salt-soluble preparation and of the alcohol-soluble 
preparation. These lime-water solutions of proteids were then 
treated with = - hydrochloric acid until the solution was neutral 
to phenolphthalein, with the following results: 
TABLE IV.—PoOWFR OF DIFFERENT PREPARATIONS TO COMBINE WITH CALCIUM. 




sii of s : z 
See: n- quivalent er ct. 
ee San between lime-| to CaO of CaO 
ie : water and combined combined 
neutralize solutions of with with 
; proteids inf proteid. proteid. § 
e-water. 
et ce. Gram. Per ct. 
GO. GG; TMe=wWa OL. catueteeeraseicns ear alee 19.5 — a — 
.5 gram of base-free casein dissolved 
in*50' ce, lime-water. ..<. . secs. 1555 4 .01120 2.24 
.5 gram of alcohol preparation dis- 
solved in 50 cc. lime-water........ 15.4 FW | .01148 2.29 
.5 gram of salt-soluble preparation 
dissolved in 50 cc. lime-water..... 15.6 3.9 -01093 - 2.19 
These results show that the salt-soluble and alcohol-soluble 
preparations made from cheese possessed the same power of com- 
bining with calcium as did casein or paracasein known to be 
base-free, and that they must therefore have been base-free pro- 
teids capable of forming a calcium combination equivalent to 
that of basic calcium casein or paracasein. These preparations 
from cheese appear therefore to be identical with base-free para- 
casein. . 
The calcium of the ash present was evidently not a part of the 
proteid molecule but was probably an impurity of calcium lactate 
or possibly this salt loosely combined with the proteid, appearing 
on ignition as calcium phosphate. 
