





243 
had been kept alive during the winter, from an inoculation (Exper. 
84) of the previous season. Five days afterward, the bacteria from 
the diseased splinter having multiplied to such an extent as to 
thickly fill the liquid, a small drop was transferred to another cult- 
ure vessel with sterilized liquid. It is evident that any substances 
that could be diffused through the liquid originally introduced with 
the splinter of wood, unless vitalized and capable of multiplication 
like the bacteria, would be so much diluted by this process that the 
drop transferred to the new culture could contain but a small per 
cent. of the amount first introduced. The second culture remained 
twenty days when a drop was transferred to a third. This was 
repeated through six cultures, and from the last, after an interval 






SERIES A. 
No. of Date of : | Amount 
Galtate. Titention. Kind of Culture Vessel. of Fluid: 
301 March 27 Salmon culture tube_______- 20 cc. 
802 April 1 Salmon culture tube________ 20 cc. 
307 April 21 Sternberg culture flask _____- +, CG. 
309 April 24 Sternberg culture flask_____- 7 PCC 
310 April 27 Sternberg culture flask______ 4 ce. 
320 June 56 Test tube with Fol stopper__- 25 CC. 
362 June 13 Bartlett pear on tree________- 


of about three months from the beginning of the series, a drop was 
introduced into a Bartlett pear on the tree. If any of the unvital- 
ized substances brought into the first culture along with the bacte- 
ria, to which the disease might be ascribed, were still to be found 
in the drop used for inoculation, the quantity was assuredly so 
minute as to be inoperative, or to induce the disease in a very mild 
form. The result of the inoculation was, however, to thoroughly 
permeate the fruit with the bacteria by the ninth day, and to break 
down the soft tissues into a viscid liquid in the characteristic man- 
ner of the blight. The above table—Series A—gives the record 
number, the time of infecting, the kind of culture vessel, and the 
quantity of culture liquid in cubic centimeters used in each succes- 
sive culture. 
Another partially independent series—detailed in table B—gave a 








SERIES B. 
No. of Date of : Amount 
Culture. Infection. Kind of Culture Vessel. of Fluid. 
301 March 27 Salmon culture tube_________- 20 cc. 
302 April 1 Salmon culture tube______.___ 20 cc. 
307 April 21 Sternberg culture flask _______- 4 ce. 
309 April 24 Sternberg culture flask __.____- 4 ce. 
314 May 22 Sternberg culture flask _______- 4 cc. 
822 June 5 Test tube with Fol stopper___-- 25 ce. 
364 July 13 Bartlett pear on tree___-_-_ 20. 


practically identical result, thus showing that it was by no means a 
matter of accident. 
