376 | 
Method D.. . 
Boil with acid one-half. hour, allow to settle, siphon. off 
fluid, add 200 cc. water, boil one-half hour, allow to settle, 
siphon off fluid putting with first, add 200 cc. water, boil 
one-half hour, let it settle, siphon off liquid with the first 
saving the whole ; to the residue in the beaker or flask add 
the alkali and repeat the operation as with acid, at last 
filtering, and having siphoned or decanted off the most of 
the liquid from the alkaline decantation return the last with 
the small amount of solids to the filter, wash until no longer 
alkaline, then add any residue from beaker containing acid 
decantation, wash the whole upon the filter until no longer 
acid, then wash with alcoholandether. They then proceed 
as in the previous ones. 
Method EH. 
A method which in print, illustrated by figures, looks 
perfection, but in practice a failure, is that with fiber bulbs. 
Thin glass globes terminating at the bottom ina funnel 
tube and open at the top to admit a cork bearing a glass 
tube and a second opening to allow of escape of steam. In 
the globe are put a platinum cone and asbestos filter, the 
whole carefully dried and weighed, then a known quantity 
of the substance to be examined is added and. 200 c.c. of 
acid solution put in and the whole kept hot by passing 
steam into the solution, after one-half hour it is filtered 
with aid of a filter pump and treated in the same manner 
with an alkali, then washed successively with water, alco- 
hol and ether. The globe, containing the fiber is now dried 
and weighed, the increase in weight is crude fiber. No 
account of ash or nitrogenous matter is taken by this 
method. 
Besides the methods tested I made a few trials to deter- 
mine the influence of the degree of heat employed in the 
fiber determinations. In the column marked F the solu- 
tions were first brought to a boil then put on the steam 
bath for the remainder of time, otherwise the same as 
method A. | | 
Those in column G were heated on steam bath alone, 
otherwise like A. The steam used for the heating came 
from the boiler supplying heat for the building at a pres- 
ure of 5 to 10 pounds. =~ ) . | 
The single determination in H was made upon the water 
bath; in other respects like A | 
. 
From former observations I thought the amount of ma- 
terial used had some influence on the per cent. of fiber 


