New York AGRICULTURAL EXPERIMENT STATION. 175 
TABLE I.—INFLUENCE OF VARYING AMOUNTS OF CHLOROFORM UPON THE 
ACTIVITY OF ENZYMES. 
In 100 lbs, total nitrogen, 
~A————- 




Nitrogen for 
Amount of Total soluble albumoses and Nitrogen in No. of 
ehioroform, Age, nitrogen, peptones. amides, germs per 
Per ct. ‘Days. Lbs. Lbs. Lbs. bed 
. fresh 9.33 4.58 4.75 
2.5 7 11.60 6.81 4.79 28 
5 7 11.63 6.94 4.69 46 
10 7 11.72 7.07 4.65 26 
20 7 11.63 6.79 4.84 25 
30 7 12.28 7.87 4.91 18 
2.5 21 16.80 9.94 6.86 42 
D ya | 16.16 8.47 7.69 36 
10 21 16.39 8.99 7.40 25 
20 21 16.37 8.34 8.03 26 
30 21 138.69 7.07 6.62 30 
235 49 21.79 14.75 7.03 
5 49 21.65 14.63 02 ——— 
10 49 21.40 14.93 6.47 ——— 
20 49 20.11 14.36 5.79 —_— 
30 49 22.99 1Get 6.82 
2.0 112 33.15 18.81 14.3: 9 
5) 112 33 .62 18.59 15.03 10 
10 112 30.51 16.38 14.13 11 
20 112 83.78 16.39 47-39 6 
30 112 33.06 19.15 13.91 5 
2.5 192 41.98 19-18 22°30 13 
5 LO 39.37 14.85 24.52 14 
10 192 35.36 15.13 20.23 T 
4 192 35.65 16.37 19.28 6 
30 192 35.78 17.30 18.48 

During 112 days the amount of soluble nitrogen varied within 
very narrow, limits in the different bottles. During the next 80 
days the bottles containing 2.5 and 5 per ct. of chloroform 
showed a little more soluble nitrogen than the others, in which 
the amounts of soluble nitrogen were almost identical. These 
results could hardly be interpreted as indicating, even after the 
lapse of 192 days, any marked difference in the effect of definite 
quantities of chloroform upon the activity of enzymes. The 
germ content, as shown by bacteriological analysis, is in entire 
agreement with the results of chemical analysis. 
From these results we see that in the presence of 2.5 per ct. 
of chloroform the increase of soluble nitrogen is continuous and 
