182 Rervorr oF THE CHEMICAL DEPARTMENT OF TUE 
TABLE LV.— Continued. 
In 100 lbs. total nitrogen. 





lars ae eee 
Germicide used. Nitrogen in 
Pe ak ee eee Total albumoses Number of 
Formalin Chloroform ; soluble and Nitrogen in germs per 
0.1 per ct. 4 per cent, Age. nitrogen. peptones, amides. cee. 
Days. Lbs. Lbs. Lbs. 
Fresh aa — a 138 
VII el Giants tains Ve 14 32.41 19.638 12.78 3 
VIII 14 00.67 29.42 Aigo 3 
VII Pa Sg oat 42 40.12 27.47 L2265 2 
Vor 42 Dt.29 29 12 Pa ees | 1 
VII @eecceeceoes rir} AT 00 29.40 17.60 0 
VIII 17 64.3 39.88 24.50 0 
VII a hake Saw tela de 152 49.91 34.37 15.54 0 
VIIl 152 62.48 39.75 23.73 3 
From the above results we see that the number of bacteria in 
all the bottles remained very low. In all cases the decompo- 
sition has gone on more slowly in the presence of formalin than 
with chloroform, as is clearly shown by the following tabulated 
Summary of results. 
TABLE 1V A.—AVERAGE OF FOUR QUARTERS, 
Total soluble nitrogen. 

Age, With formatin. ‘owich ahisrateeies 
Days. Per ct. Per ct. 
14 32.15 47.26 
42 39.07 55.39 
co 46.00 62.10 
152 45.62 60.63 
In the article by Jensen previously referred to, he notes the 
same relation in the action of these two substances and he is 
inclined to hold the view that 0.1 per ct. formalin completely 
inhibits the action of galactase but allows bacterial enzymes 
to work. If this view is correct, we must consider that over 70 
per ct. of the decomposition here produced in the presence of 
chloroform is caused by enzymes other than galactase. 
It seems hardly possible that sufficient bacterial enzyme could 
have been formed in the cases of No. III to account for the 
changes observed in the presence of formalin. The milk in this 
quarter of the udder was unusually free from bacteria, having 
