New York AGricuLtuRaAL EXPERIMENT STATION. 185 
meter; the back left quarter had slightly less; the front Icft 
quarter had often less than 100 per cubic centimeter, and the 
front right quarter but little more. 
Making allowance for the work done by galactase, the milk 
from different quarters of the udder of the above mentioned cow 
should show different rates of chemical change proportional to 
the number of germs present in the respective quarters of the 
udder, if these changes are to be associated with contamination 
within the udder. 
The results already given in Table IV, under chloroform, 
relate to this point. The quarters of the udder are there 
designated as follows: II, front right; IV, front left; VI, back 
right; VIII, back left. The second determination was made in 
the presence of 4 per ct. of chloroform. In order to obtain 
sufficient material for a large number of analyses, three sue: 
cessive messes of milk were collected and united. Care was 
taken to reject the fore-milk and keep out bacteria from other 
sources. The following table shows the results in this test up 
to 15 weeks: 
TABLE VY B.—SOLUBLE NITROGEN FORMED IN MILK FROM DIFFERENT 
QUARTERS OF UDDER. 
MILK DRAWN JUNE 11 AND 12. 
Soluble nitrogen in 100 lbs. total nitrogen. 
AGE OF MILK —————— 
WHEN ANALYZED. Front left Front right Back left Back right gy 
quarter. quarter. quarter, quarter, 
Days. Lbs. Lbs. Lbs. Lbs, 
7 27.01 748 Pas 48 .23 46.66 
21 36.25 39.75 56.61 56.66 
35 36.65 40.60 59.37 57.54 
49 lost 40.48 60.68 59.63 
105 lost 55.31 Cls20 71.638 
The results given in Tables IV and Vp show in a general 
way that there is a relation between the numbers of bacteria 
present in the udder and the rapidity with which the milk pro: 
duced there undergoes self-digestion in the presence of chloro. 
form ov formalin. 
It may be held that the presence of these bacteria has merely 
stimulated the production of an extra amount of galactase, but 
