New York AGRICULTURAL ExpERIMENT STATION. 743 
September, occurring in some localities and not in others. If the 
dry spell continued long enough the disease would appear in other 
_ fields, which, of course, was an indication of its spreading. 
Artificial cultures of the disease have been obtained by Dr. 
Forbes, of Llinois, and at the Iowa Expariment Station. Inocu- 
lations have been made from these cultures in both of the States, | 
but in every case the appearance of the disease in the fields has 
interfered with carrying the experiments far enough to obtain 
positive results. Still another cause which has interfered with 
the determination of the contagious character of the disease is 
the fact that no resting spores of this J/tcrococcus have been 
obtained in artificial cultures. During the fall of 1893, at the 
Iowa Experiment Station, a number of the diseased larvee were 
obtained from the field and placed in adry atmosphere until they 
became mummified. The past season Mr. Charles W. Malley, of 
the above Station, made cultures from these mummified larve. 
As far as microscopical characters and method of growth on 
culture media could be relied upon, the pure J eerococcus of the 
cabbage-worm disease was obtained. Some of the above cultures 
were obtained from Mr. Malley and bouillon, or beef tea, cultures 
were made from these. On August 22 a rieos of larve of 
Pieris rape and of the cabbage Plusia were taken to the labor- 
atory from the field of Mr. Van Dine and placed on cabbages grow- 
ing in flower pots. The bouillon was sprayed on the leaves and 
larve. By August 27 all the larve of the imported cabbage 
worm were dead, while Plwsza did not seem to be affected in the 
least. In a second case the bouillon was sprayed on the cabbage 
and the larye placed on the plants afierward. On August 27 
all these larvee were dead except one which had pupated. In the 
first case the bouillon culture was sprayed on the plants, full 
strength; in the second it was diluted with water 10. parts te 
one of the culture. 
On August 29 when Mr. Van Dine’s cabbage field was revis- 
ited to obtain a new lot of material to work with, the disease was 
_ found raging at a frightful rate. This of course puta stop to 
work with material from that field and deprived the test of all . 
certainty. 
A second attempt was made September 12 on material ob- 
tained from Mr. Van Dergau’s near Queens, L. I. The old 
