NEw York AGRICULTURAL EXPERIMENT STATION. AI 
The results are given in pairs tepresenting the duplicate bot- 
tles. In this experiment 0.1 per ct. of chloroform did not hold 
the germs in check, although at first it destroyed a portion of 
them. These bottles curdled after two and four days respec- 
tively. Apparently, the chloroform entered into some combina- 
tion with the solids present, so that the growth was not pre- 
‘vented, as is shown by the high germ content at the end of a 
week. Before three months the number fell to as low a point as 
in the other bottles, probably on account of the accumulation of 
decomposition products. The germ content in the bottles con- 
taining 0.3 per ct. and more of chloroform fell promptly to a 
point that undoubtedly represented little, if anything, more than 
spores present. The bottles containing 0.3 per ct. or more of 
chloroform all curdled after about two months with a curd 
characteristic of enzym action. 
These results were tested by a second series of bottles. 
The milk used in this experiment was twice passed through 
the centrifugal separator and the fat reduced to 0.015 per ct. 
The milk contained 408,500 germs per cc. Chloroform was added 
to duplicate, well-stoppered bottles at the rate of 0.1, 0.2, 0.3, 0.5, 
0.7, 1.0, 1.5, 2.0, and 2.5 per ct. The bottles were kept at 21° C. 
(60° F.). The numerical results of cultures made at the end of 
one, three, and six months are given in the following table. 
Taste III].— Germs PER Cusic CENTIMETER IN CHLOROFORMED SKIM-MILK.* 


PERCENTAGE OF CHLOROFORM BY VOLUME, 





INTERVAL. 
0.1 Meza Oso ANG Oo IMO er lea On [dace c ON erarO 
UBC e rs eRe chs sft BAs Wiahe ea ss OUTPAedie waaes 485. 35. (c86) | SOCh Sa5ln4Ont a5 46 
e 44.; 35 | 50.| 15} 25 |} 48 | 44 42 
Kol prataith a) Mel B se a8 a ae a Too many SOP TOON) SOL 2> 4 LO 26 iad’ 20 
to count AS |e GONE OS We oie LO) feted 26 3 
(o: gaY aS. ek cw a LO Ae Oa a 189 ,000 ie US ed 20} LOS Deo: FTO 13 
SE SE ee 34 | 14 6 8 4} 12 th 


*Determinations made by Mr. Nicholson. 
This table shows that while 0.1 per ct. of chloroform was not 
sufficient to preserve this skim-milk, 0.2 per ct. reduced the germ 
content as successfully as a larger amount. It should be noted, 
however, that the first determination was made after an interval of 
- one month. 
