42 Report OF DEPARTMENT OF BACTERIOLOGY OF THE 
The bottles containing 0.1 per ct. of chloroform curdled after 
four and seven days respectively with a curd characteristic of 
acid action, while all the remaining bottles curdled after slightly 
less than two months with a soft curd, characteristic of enzym 
action. 
In considering the action of a germicide in connection with 
enzym studies, it is important to know the rate at which the 
germs present in the digesting solution are destroyed, as well as 
the final limits of the destruction, since it is conceivable that in 
the interval sufficient enzym may be elaborated to affect the 
results markedly. 
Accordingly, in December, 1906, an experiment was carried out 
in which duplicate bottles of milk, which had been twice passed 
through the centrifugal separator to remove the fat more com- 
pletely, received chloroform at the rate of 0.1, 0.2, 0.3, 0.4, and 0.5 
per ct. respectively. The results of subsequent examinations are 
given in Table IV. 
TABLE IV.— GERMS PER CUBIC CENTIMETER IN CHLOROFORMED SKIM-MILK.* 
| SS ERE TRE RSS A ES Se a SE SANE TS AEE EEE EE ST SME RIC LL DS ESS EEN STORE eS SURES cee SESE SET 


- PERCENTAGE OF CHLOROFORM BY VOLUME, — 
INTERVAL. ee OS 
0.1 OP Se) is O88 0.4 0.5 
Injitial content 166 ,000 
OGhOULS ae ei rdi enc ie AR SO. 210 ,000 177 ,000 60 ,000 10,200 5,300 
290 ,000 205 ,000 81,000 18 , 200 4,000 
PARC UTS A aos es Voce elchen a, cue aeae Too many 85,200 11,400 280 45 
to count 54,000 18,500 520 220 
RSUCECLN Say chetene) 21. Aur Se ene Pe Ne 250 ,090 21,500 4,800 20k 120 
324,000 27,000 8,100 95 500 
PUGS ee OLA SEEN ees A 6,900 2,300 24 23 
Fe Oe ee 16,700 2,700 18 |} cyl teeeee 



| rae DST SERS SEL SEE EE SF I ES TS ESO SE NAB Eo 
* Determinations made by Mr. Prucha. 
From these results it will be seen that all amounts above o.1 
per ct. produced a steady decline in the germ content of the 
milk. In the case of 0.4 and 0.5 per ct., this decline was so 
sharp that within twenty-four hours the numbers present could 
hardly represent more than the spore content of the milk. Ia 
the case of 0.2 and 0.3 per ct., the decline, while evident, was 
so gradual that at the end of eleven days vegetative forms were 
still present in comparatively large numbers. 
From the above results, it would seem evident that while 
bouillon with 0.5 per ct. of proteid was at once protected from 
