New YORK AGRICULTURAL EXPERIMENT STATION. 43 
germ action by the presence of o.1 per ct. of chloroform, skim-+ 
mill with 3 per ct. of proteids required 0.4 per ct. of chloroform 
to give it an equivalent protection. 
EFFECT OF FAT UPON THE GERMICIDAL VALUE OF CHLOROFORM. 
Babcock and Russell (1) found that while 15 per ct. of ether 
would preserve milk containing 4 per ct. of fat, heavy cream 
required an equal volume of ether in order to prevent germ 
action. In this case the fat appears in some way to reduce mark- 
edly the germicidal value of the ether. The effect of fat upon 
the action of chloroform has been studied in the following ex- 
periments. | 
Sper pct. Jol fatececrived 
of chloroform respectively and were 
Bottles of normal milk containing 
ay We24anid 0.3, per ct. 
Gop a?  C. (99° F.). 
TABLE V.— GERMS PER CUBIC CENTIMETER IN CHLOROFORMED MILK 
TAINING 5 PER CT. OF FatT.* 
poe a ma ee Sasi See = = a 
Con- 









PERCENTAGE OF CHLOROFORM BY VOLUME. 
INTERVAL. 
Ort Oe, 0.3 
NDS! 2) CS ey Se a ee 780 ,000 | 313,000 720,000: 
LL GEES 24, 6 ane SU) i a ee eae 97 .000 222 ,000 255,300) 
hy HEE 7 ce a rr ae 147 ,000 | 390 ,700 212,000: 
MIR eek. ns blew sl eee Hse 583 ,000 2,884,000 447 ,000: 
ih Na i 5,245,000 18:312. 000.) Too -manw to 
count 
CM mini! eys4).). ic chife ls Se dhw see eee | 451,970,000 | Too many to | Too many to 
| count count 
ei NE Sn ee Too, many ‘to | Too, many sto, | ‘Too many’ to 
count count count 
Ghose Oe Ae ee ie. a re Curdled Curdled | Curdled 
| ae a = — St 



*Determinations made by Mr. Nicholson. 
These results show that 0.3 per ct. of chloroform is not sufficient 
to saturate 1 per ct. of fat in addition to the other solids and leave 
enough excess to destroy the germ life. Accordingly, further tests 
were made with larger proportions of chloroform. 
In this case milk was obtained from the cow under such pre- 
cautions that the germ content was only 31 per cc. Duplicate 
bottles received 0.3 and 0.5 per ct. of chloroform and were held 
at 37° C. (99° F.). The two bottles which contained 0.3 per ct. 
curdled on the second day, while both bottles containing 0.5 per 
ct. curdled on the third day. 
