‘ 
46 Report OF DEPARTMENT OF BACTERIOLOGY OF THE 
RATE OF GERMICIDAL ACTION IN .MILK. 
From the data already given, it is evident that the destruction 
of bacteria by chloroform is not an instantaneous matter, but 
rather a progressive one, being dependent upon the amount of 
chloroform which is active, and upon the length of exposure to 
its influence. Under such circumstances, it is conceivable that, 
while the chloroform might ultimately destroy the germs in the 
milk, it might do this so tardily that in the interval there would 
be sufficient enzym formed to produce observable changes in the 
compounds present. In order to obtain more exact information 
of the rate at which the germs were destroyed by different per- 
centages of chloroform, duplicate series of bottles were prepared, 
containing normak milk with 5 per ct. of fat and varying 
amounts of chloroform. Each bottle contained 300 cc. and was 
held at approximately 22° C. (72° F.) during the period of ex- 
amination. Plates upon lactose agar were made in triplicate at 
short intervals and the results from these plates are given in 
thable VIL. | 
TABLE VIII.— GerMS PER CUBIC CENTIMETER IN CHLOROFORMED MILK COoNn- 
TAINING 5 PER CT. OF Fat.* 























PERCENTAGE OF CHLOROFORM BY VOLUME, 
INTERVAL. 
0.0 0.5 1.0 Avs 2.0 2.5 
Lay Pin | 34.600 24,500 | 33,200 | 19,800 | 12,000 O+ 
41/300 27'500 | 24500 | 16/000 5/980 O+ 
BG eran aes ieee tek 36, 700 27,600 | 23,100 3,500 796 | 20 
39000 28800 | 18800 5.290! - 9731 30 
LAUR Y Oa aD 10,000 67,700 6.810 1,360 731 33 
1187800 53.300 9980 1/710 163 | 114 
GE aR an 112.800 28 , 700 6, 430 795 . 851 43 
| 138500 26/900 | 11210 1,220 90 | 44 
Louver Mindd ba he 5,070 ,000 143,000 4,100 400 38 | 641 
4°145'000 151,200 | . 3'650 354 98 | 591 
ie RT Ie de CT 2 042,000 3,540 {ih 33. | 37 
Sepp sty ae 1'585.000 3090 242 32 | 25 
Bay toa tren HL Tue aan ‘| 6,813,000 |’ 3,436 48 31 | 21 
5' 86/000 3/480 75 is | 27 
ss ROC ae curdied | 45,467,000 1,360 66 29 | 27 
«“ 43600000 1'100 123 43 | 19 



* Determinations made by Mr. Prucha. _ u ; 
} No colonies appeared upon plates with dilutions of 1-70, i-200 and 1-1000 respectively. 
The results are given in pairs representing the duplicate bot- 
tles.. The two control bottles increased in germ content so 
rapidly that the plates made at the end of 22 hours and subse- 
quently were too thickly seeded to be counted satisfactorily. 
